Protein Info for CA265_RS09440 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 TIGR01063: DNA gyrase, A subunit" amino acids 13 to 815 (803 residues), 1178 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 37 to 475 (439 residues), 586.4 bits, see alignment E=3.8e-180 PF03989: DNA_gyraseA_C" amino acids 509 to 553 (45 residues), 41.8 bits, see alignment 5.7e-15 amino acids 558 to 600 (43 residues), 29.4 bits, see alignment 4.2e-11 amino acids 619 to 656 (38 residues), 20.3 bits, see alignment (E = 3.1e-08) amino acids 674 to 714 (41 residues), 37.1 bits, see alignment 1.7e-13 amino acids 720 to 763 (44 residues), 47.8 bits, see alignment 7.8e-17 amino acids 769 to 814 (46 residues), 49 bits, see alignment 3.2e-17

Best Hits

Swiss-Prot: 52% identical to GYRA_STAES: DNA gyrase subunit A (gyrA) from Staphylococcus epidermidis (strain ATCC 12228)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 84% identity to phe:Phep_2163)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z4G7 at UniProt or InterPro

Protein Sequence (863 amino acids)

>CA265_RS09440 DNA gyrase subunit A (Pedobacter sp. GW460-11-11-14-LB5)
MAEDLENQQNDKIIQINIEEQMKSAYIDYSMSVIVSRALPDVRDGLKPVHRRVLYGMLDL
GVTSNKPHKKSARIVGEVLGKYHPHGDASVYFTMVRMAQDWSLRYPMVDGQGNFGHIDGD
SPAAMRYTEARFSKIAEEMLADINKDTVDFQLNFDDTLQEPTVLPAKIPNLLVNGSSGIA
VGMATNMAPHNLTEVIDGVVNYIDNRDITIEELMKFVKAPDFPTGGIIYGYTGVKEAFET
GRGRIVMRAKAEIESIKDREVIIVTEIPYQVNKAQMIERTAELVGEKKLEGISNIKDESN
KDGIRIVYEIKRDANASIVLNNLYKYTALQTSFSVNNIALVKGRPQMLNLKDLIVHFVDH
RHDVIVRRTRYELAEAEKRAHILEGYLIALDHLDEVIKLIRASETPEDARLGLMEKFGLS
DIQARAILDMTLRRLTGLERDKIKEEYAELMKTIEHLKSILADEGLRMQIIKDELAEIRQ
KFGDERRTTIVHSAEDMSMEDFIDDEEVVITISHEGYVKRTPATEFKAQGRGGKGSKGST
SRNEDFIEHMLVATAHNYMLFFTEAGRCFWLRVYEIPEGSRTSKGRAIQNIINIPKEEKI
KAYIKVKNLKDQEYLENNFIIMCTKKGTIKKTSLEAYSRPRVNGINAININDGDVLLEAC
LTNGESEIVMALRSGRAIRFNESTVRPMGRTATGVRGVRLAHDQDEVVGMIAVNNPEVTV
LVVSEKGYGKRTDIEDYRVTNRGGKGVKTINVTEKTGQLVSIKDVTDSEDLMIINRSGIV
IRIPVSALRVMGRATQGVRLISLKGDDEIASVTKIDHEEDEEETVDLTDVVVTDGEEVEA
DTTPETDDTEEEADSDNETEEEN