Protein Info for CA265_RS08950 in Pedobacter sp. GW460-11-11-14-LB5
Annotation: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to FOLD_BACFR: Bifunctional protein FolD (folD) from Bacteroides fragilis (strain YCH46)
KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 88% identity to phe:Phep_2069)MetaCyc: 52% identical to methylenetetrahydrofolate dehydrogenase [multifunctional] (Bacillus methanolicus)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]
Predicted SEED Role
"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)
MetaCyc Pathways
- folate transformations III (E. coli) (8/9 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
- formaldehyde oxidation VII (THF pathway) (4/4 steps found)
- folate transformations II (plants) (9/11 steps found)
- tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
- formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
- L-histidine degradation III (3/6 steps found)
- folate transformations I (8/13 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
- purine nucleobases degradation II (anaerobic) (15/24 steps found)
- reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
- purine nucleobases degradation I (anaerobic) (5/15 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.5.1.5 or 3.5.4.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1X9Z3S7 at UniProt or InterPro
Protein Sequence (306 amino acids)
>CA265_RS08950 bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase (Pedobacter sp. GW460-11-11-14-LB5) MSDGNSKSPSGDLGVLLDGKYVSEKVKVQIAEEAAEFLNKSGRKPHLVAILVGNDGGSET YVASKMKNCEKVGFKSSLHRYDNSVTEAELLAKIEEINQDDEVDGLIVQLPLPKHIDPEK VTEKIDHRKDVDGFHPVNLGRMMRNLPCFIPATPYGILLMLQEYGIDTTGMHCVVVGRSN IVGSPMSILMARNANPGNCTVTLTHSRTKDLKEQVLQADIVIAAIGKKNFVTADMVKPGA IIIDVGINRETSAETKSGFKLYGDVDFENVAPKASYITPVPGGVGLMTIVGLLKNTLASA RKEIYA