Protein Info for CA265_RS08825 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 PF01745: IPT" amino acids 5 to 54 (50 residues), 23.5 bits, see alignment 3.5e-09 TIGR00174: tRNA dimethylallyltransferase" amino acids 6 to 290 (285 residues), 187.6 bits, see alignment E=1.5e-59 PF01715: IPPT" amino acids 39 to 276 (238 residues), 182.3 bits, see alignment E=1.3e-57

Best Hits

Swiss-Prot: 45% identical to MIAA2_BACFR: tRNA dimethylallyltransferase 2 (miaA2) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 58% identity to phe:Phep_2050)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.75

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z3G1 at UniProt or InterPro

Protein Sequence (309 amino acids)

>CA265_RS08825 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Pedobacter sp. GW460-11-11-14-LB5)
MDQHNLIIILGATASGKTKLAVSVAEALNGEIISADSRQIFKRMDIGTGKDLQEYHINGK
TIPYHLIDILEPGERYHVDAFKNDFYQAFASITAKGKIPILCGGTGMYIHSLLQNQTFTA
IPVNQQLRDQLNLLSKEELISKMHGDKSQNSDHVDLSSKKRLIRALEIADYLKHNKLETV
ERPKIKPIIFGLKNEVEVTRAKILSRLDERFKAGLIDEVAQLLKDGISKEILVFYGLEYK
FIVNYLDGLFTFEELRLKLGIAICQYAKRQNTFLRKMEKDSVEIIWLDASAPVNHLQQLI
LQKVSVIQN