Protein Info for CA265_RS08625 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): Ribulokinase (EC 2.7.1.16)
Rationale: Specifically important for utilizing L-Arabinose. Automated validation from mutant phenotype: the predicted function (RXN0-5116) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: ribulokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01234: ribulokinase
  • amino acids 6 to 558 (553 residues), 593.5 bits, E=2.1e-182
• PF00370: FGGY_N
  • amino acids 6 to 283 (278 residues), 30 bits, E=4.1e-11
• PF02782: FGGY_C
  • amino acids 293 to 508 (216 residues), 129.1 bits, E=2e-41

Best Hits

KEGG orthology group: K00853, L-ribulokinase [EC: 2.7.1.16] (inferred from 72% identity to phe:Phep_2729)

Predicted SEED Role

"Ribulokinase (EC 2.7.1.16)" in subsystem L-Arabinose utilization (EC 2.7.1.16)

MetaCyc Pathways

• L-arabinose degradation I (4/4 steps found)
• D-arabinose degradation I (1/2 steps found)
• ribitol degradation I (1/2 steps found)
• D-arabinose degradation V (1/3 steps found)
• superpathway of pentose and pentitol degradation (12/42 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.16

Sequence Analysis Tools

See A0A1X9Z3B1 at UniProt or InterPro

Protein Sequence (566 amino acids)

>CA265_RS08625 Ribulokinase (EC 2.7.1.16) (Pedobacter sp. GW460-11-11-14-LB5)
MSTANYVIGVDYGTDSVRSVLVDTANGKEIASSVFLYPRWQKGLYCKPAVNQFRQHPLDY
IEGLTHTIKDCLAKAGGAEIAHLVKGISVDTTGSSPVAVDATGTPLALTKDFEENPNAMF
VLWKDHTSVKEAAEINEHATKFDTNYLKYVGGIYSSEWFWSKLLHILRVDLTIKKGAASW
VEHCDWIPFLLCGGNDISTMKRSRCAAGHKALWAEEFNGLPPEDFFSSLDPLLAGFRAKL
FTDTYTSDVSAGTLSEEWANKLGLNTDVVVGVGAFDAHMGAVGGQIEPYYLSKVMGTSTC
DILVAPNQDLHGKLINGICGQVNGSVIPGMAGLEAGQSAFGDVYAWFKNLISWPLNHLLT
ESEVIDEATATALKAELEGKIIANLSKQADALPNEDYAELAIDWLNGRRTPDANQELKGA
ITGLGLGTDAPRFFRALAAATCFGAKAIVDRFKEQGVPVKGIIGIGGVAKKSAYIMQMMA
DVLEMPIRIHRFEHTCALGAAMFAAVAAGIYPDIETAMAAMGTGFEKEYKPNIKKQKLYR
QHYQQYLGLGRYLEKYNKKDVKPYLS