Protein Info for CA265_RS08615 in Pedobacter sp. GW460-11-11-14-LB5

Updated annotation (from data): L-arabinose isomerase (EC 5.3.1.4)
Rationale: Specifically important for utilizing L-Arabinose. Automated validation from mutant phenotype: the predicted function (ARABISOM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: L-arabinose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF02610: Arabinose_Isome" amino acids 5 to 359 (355 residues), 601.5 bits, see alignment E=6e-185 PF11762: Arabinose_Iso_C" amino acids 362 to 475 (114 residues), 167.3 bits, see alignment E=9.7e-54

Best Hits

Swiss-Prot: 64% identical to ARAA_HERA2: L-arabinose isomerase (araA) from Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95)

KEGG orthology group: K01804, L-arabinose isomerase [EC: 5.3.1.4] (inferred from 85% identity to phe:Phep_2731)

MetaCyc: 55% identical to L-arabinose isomerase (Escherichia coli K-12 substr. MG1655)
L-arabinose isomerase. [EC: 5.3.1.4]

Predicted SEED Role

"L-arabinose isomerase (EC 5.3.1.4)" in subsystem L-Arabinose utilization (EC 5.3.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z3B4 at UniProt or InterPro

Protein Sequence (496 amino acids)

>CA265_RS08615 L-arabinose isomerase (EC 5.3.1.4) (Pedobacter sp. GW460-11-11-14-LB5)
MIDLKKLQVWFITGTQHLYGEETLKQVAAHAQEVADSLNQNGSISVSVVYKPIVKTTEEI
FETLQQANIDENCIGVITWMHTFSPAKMWIRGLNVLQKPLLHLHTQFNRDIPWNTIDMDF
MNLNQSAHGDREFGFMVSRMRKDRKVVVGHWQDEEVAKQIDTWCRAAAGWHDWQGAKFAR
FGDNMRYVAVTDGDKVEAEMKFGFAVNTYGIGDLVAVINGIGEDAIQSLLEEYEATYEMA
DDLKAGGARHSSVYEAAKIELGLRKFLVDGGFKGFSDTFEDLHGMIQLPGIAAQRLMADG
YGFAGEGDWKTAALVRACKVMGAGLAGGNAFMEDYTYHFDPANSMVLGSHMLEVDASLAS
GKASLEVHPLGIGGKADPARLVFNVAGGDALNAALIDMGNRFRLLVNEVKAVEAEHDLPN
LPVARVLWKPLPDMKTGCAAWIYAGGAHHTAYSQNLTTEHLLDFANIAGLEYVNIGADTK
INQFRNELHWNEVFYK