Protein Info for CA265_RS07505 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 349 (349 residues), 408.4 bits, see alignment E=1.1e-126 PF02547: Queuosine_synth" amino acids 3 to 348 (346 residues), 455.4 bits, see alignment E=5.5e-141

Best Hits

Swiss-Prot: 74% identical to QUEA_FLAPJ: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 94% identity to phe:Phep_2572)

MetaCyc: 49% identical to S-adenosylmethionine--tRNA ribosyltransferase-isomerase (Clostridioides difficile)
RXN0-1342 [EC: 2.4.99.17]

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.99.17 or 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9ZCB2 at UniProt or InterPro

Protein Sequence (349 amino acids)

>CA265_RS07505 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA (Pedobacter sp. GW460-11-11-14-LB5)
MKLSQFKFNLPESLVANNPAEQRDEARLMVLHKDSGKIEHKIFKDVLGYFDDKDVMILNN
TKVFPARLYGNKEKTGATIEVFLLRELNKELRLWDVLVDPARKIRVGNKLYFGDDDLLVA
EVVDNTTSRGRTIRFLFEGTDEEFRKNVEILGETPLPKYIKRKATAEDKERYQTIFAKHE
GAVAAPTAGLHFSRELMKRLELKGVEFAEVTLHVGLGTFRTVEVEDLTKHKMDSEQFIIE
QKDANIVNKALEEKRKICAVGTTSMRAIESAVSANRTLKAANDWTSKFIFPPYDFSIANS
MITNFHTPESTLLMMVSAFGGYENVMNAYEVAVKEKYKFYSYGDAMLII