Protein Info for CA265_RS06225 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 12 to 446 (435 residues), 399.2 bits, E=1.1e-123
• PF00206: Lyase_1
  • amino acids 14 to 313 (300 residues), 175.7 bits, E=1.6e-55
• PF08328: ASL_C
  • amino acids 330 to 445 (116 residues), 164 bits, E=1.3e-52

Best Hits

Swiss-Prot: 51% identical to PUR8_LEGPC: Adenylosuccinate lyase (purB) from Legionella pneumophila (strain Corby)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 81% identity to phe:Phep_2933)

MetaCyc: 52% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)
• dZTP biosynthesis (4/5 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Sequence Analysis Tools

See A0A1X9Z215 at UniProt or InterPro

Protein Sequence (448 amino acids)

>CA265_RS06225 adenylosuccinate lyase (Pedobacter sp. GW460-11-11-14-LB5)
MNLTSLQAISPVDGRYRKTTESLAAYFSEEALIKYRVFVEIEYFIALCEINLPGLENFDR
NLYAELRKIHENFSEVDALEIKETEKVTNHDVKAVEYFIKSKFDALSLQNYKEFIHFGLT
SQDINNTAIPYSIKLALNDSYYPAINTLIEKINALAIEWKDIPMLAHTHGQPASPTKLGK
EFLVFAERLAIQVESLKNIPNSAKFGGATGNFNAHHIAYPEVNWVNFSNQFVNETLGLTR
AQHTTQIEHYDQFAAQCDALKRINNIIIDLDRDIWTYISKNYFKQKIKAGEIGSSAMPHK
VNPIDFENAEGNAGIANALFEFFAAKLPISRLQRDLTDSTVLRNVGVPFGHTLIAINSTL
RGLNKILLNEAALHADLDENWAVVAEAIQTVLRRENYPNPYEALKELTRTNSKINAASIA
EFIEGLAVSEAVKVQLRTITPFNYTGVF