Protein Info for CA265_RS04225 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: mandelate racemase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 41% identical to ENOF1_HUMAN: Mitochondrial enolase superfamily member 1 (ENOSF1) from Homo sapiens

KEGG orthology group: None (inferred from 77% identity to dfe:Dfer_1860)

MetaCyc: 40% identical to L-galactonate dehydratase (Trichoderma reesei)
RXN-11380 [EC: 4.2.1.146]

Predicted SEED Role

"L-fuconate dehydratase (EC 4.2.1.68)" in subsystem L-fucose utilization temp (EC 4.2.1.68)

MetaCyc Pathways

L-fucose degradation II (3/5 steps found)
L-fucose degradation III (5/8 steps found)
D-galacturonate degradation IV (2/5 steps found)
superpathway of microbial D-galacturonate and D-glucuronate degradation (18/31 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.146 or 4.2.1.68

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z125 at UniProt or InterPro

Protein Sequence (406 amino acids)

>CA265_RS04225 mandelate racemase (Pedobacter sp. GW460-11-11-14-LB5)
MINKIEISDKRFELSTGAGSDAIHKDPQYSYAVTNLTNENGITGTGLAFTLGAGNDLVCN
AAQFYANKLKGQDIEELMSDFGQTFRTLSNEQQFRWLGPHKGVVHLGLASVTNACYDLWA
KKRGVPLWKLLIDLSPEEIVNTLDLSYLEDVLTKEEAIAMLQSQTDSKKSREGILDTGYP
GYDTSVGWFNYDDEKVRENCKKAIANGFTAMKLKVGSADPKRDIRRANIVREVAGESSKV
MLDANQQWTLPQAISICNELKNMNPFWVEEPTHPDDVLAHQTLAREIAPVKLALGEHVPN
RIIFKNYLQTGCTGFAQVDAVRVGGVSEFITISLLCKKFGVPVVPHVGDMGQLHQHLVLF
NHIAMGHEALFLEHIPHLKQHFKHPIKIEKGVYITPQEAGSSCDLK