Protein Info for CA265_RS04155 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: bifunctional alpha,alpha-trehalose-phosphate synthase (UDP-forming)/trehalose-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 PF00982: Glyco_transf_20" amino acids 2 to 458 (457 residues), 508.5 bits, see alignment E=2.7e-156 TIGR00685: trehalose-phosphatase" amino acids 490 to 721 (232 residues), 122.9 bits, see alignment E=2e-39 TIGR01484: HAD hydrolase, family IIB" amino acids 492 to 688 (197 residues), 84.4 bits, see alignment E=1.2e-27 PF02358: Trehalose_PPase" amino acids 493 to 691 (199 residues), 155.8 bits, see alignment E=1.6e-49

Best Hits

KEGG orthology group: None (inferred from 70% identity to phe:Phep_2229)

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15) / Trehalose-6-phosphate phosphatase (EC 3.1.3.12)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15, EC 3.1.3.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.15 or 3.1.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z1T9 at UniProt or InterPro

Protein Sequence (729 amino acids)

>CA265_RS04155 bifunctional alpha,alpha-trehalose-phosphate synthase (UDP-forming)/trehalose-phosphatase (Pedobacter sp. GW460-11-11-14-LB5)
MKTVIISNRLPVKIIEENDEYTFIPSEGGLATGLGDVYKSGNSIWIGWPGIEVPEERQEE
VTQKLAELNLYPVFLTQNEINLYYEGFSNEILWPVFHYLVTYAHYEQNYWDCYRSVNEKF
AKSAMQVLNRKDKVWIQDYQLLLLPGILREKLPNATIGFFQHIPFPSYEIFRLIPWREEL
LNGLIGADLIGFHTFDDVRHFISTATRLLPVNSSSNILSSNERQIVVEAFPMGIDYNKFS
GLTATEEVQKEIAYFRSGKEHTKVILSIDRLDYSKGILERLKALELLLQHHPEYIGKIEL
FMIVVPSRDTVPKYSELRDQIDQFVGNLNARYRSMNWIPVNYFYRSFPIEVLSALYSTAD
ICLVTPMRDGMNLVSKEYVASRNNNDGVLILSEMAGASKELTDAVIVNPNNLGDIMEAIV
TALKMPLAEQKQRMKAMRAIVEKFNVKHWVNNFILRLNEVKDSQKSLLTKHAVNAINEVI
ASQYAKTQDRVLFLDYDGTLVDFTGNIDDASPDAELYDLIENLTLDKANKVVIISGRRNE
TLEKWFGHLKVDLIAEHGAWQKSFEDKWNSLPLLTDQWKQEIKTLLETYTDRTPGSFIEE
KSYSLVWHYRKAEEGLGDLRANEIISHMKILAADKGLQLMPGNKVIEFKNMEVNKGKAAL
NWLYNKKPDFILALGDDHTDEDIFRALPDDAFTIKVGNNISEAKYYLNDFKEVRKLLWSL
VKEEFVGRS