Protein Info for CA265_RS04150 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF19291: TREH_N" amino acids 10 to 156 (147 residues), 42 bits, see alignment E=6.6e-15 PF00723: Glyco_hydro_15" amino acids 217 to 582 (366 residues), 178.2 bits, see alignment E=2.9e-56

Best Hits

KEGG orthology group: None (inferred from 77% identity to phe:Phep_2230)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z1J9 at UniProt or InterPro

Protein Sequence (594 amino acids)

>CA265_RS04150 glycosyl hydrolase (Pedobacter sp. GW460-11-11-14-LB5)
MAEKHLYQTGIIGNCAFIAHVNKNTDISWLCWPRFDSPFVFGSLLDEKKGGEFSILPQGE
FTSHQYYIENTNVLRTEITAEDGKYRITDFAPRFHLYDRYFKPLMFIRKIEPLEGSPRIT
IKCKPVCDYGKGKMKSSRGSNHIDYLGCDENIRLSTNVSLTYIFDEKAFVLNEAKYLIMT
YGQNLEAPVVSTAENFLRETIAYWRLWIKHSSIAGFYQPFVIRSALVLKIHQYEDTGAII
AASTTSLPESPGSTRNWDYRYCWLRDSHYVLTSLNHIGHFEEMERYFNYLSDISHAEDIR
YQPLYGIAGEREITERTLDHLTGYKGEQPIRVGNQAYEHIQNDIYGQVLISMLPLYTDHR
FVFSERSDSVRWIESVLSKIERTIDEKDAGIWEFRNIANVHCYSNLFQWAGAQAALKMAK
TIGNDDFEKRAQVLIDKAAAHIEACYDPERKVYTNAVGSPHLDASTLQLIMMNYLDPASD
RAKDHLIALEKELKTEDGLFYRYLHADDFGKPKTTFLICAFWYVEALACVGRTDEAIKEF
ENIIKYCNHLLLFSEDVDAKTGSQWGNFPQAYSHVGLMNAAYRIAIKLDRPIFL