Protein Info for CA265_RS01980 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 21 to 481 (461 residues), 485.9 bits, see alignment E=6.9e-150 PF01225: Mur_ligase" amino acids 23 to 95 (73 residues), 33.8 bits, see alignment E=5.3e-12 PF08245: Mur_ligase_M" amino acids 109 to 304 (196 residues), 165.5 bits, see alignment E=2.3e-52 PF02875: Mur_ligase_C" amino acids 326 to 411 (86 residues), 77.3 bits, see alignment E=1.3e-25

Best Hits

Swiss-Prot: 59% identical to MURE_CYTH3: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 79% identity to phe:Phep_3330)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9Z0P0 at UniProt or InterPro

Protein Sequence (485 amino acids)

>CA265_RS01980 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Pedobacter sp. GW460-11-11-14-LB5)
MQLQDLLYGVTIKELVGKTDREINALNFDSRKVGKDDVFFAIVGTLADGHQFIGQTIQQG
AAVIICENLPEIQDFTITYIKVENTSVALGIIAGNYFGNPSADLKLIGITGTNGKTTIAT
ILFKLFKDLGYKTGLLSTVENYINDTVVAATHTTPNPIALNQLLREMVDAGCDYCFMEVS
SHAVSQHRIEGLTFSGGVFSNLTHDHLDFHKTFDSYLKAKKAFFDVLPKSAFALTNIDDK
NGVVMLQNTKAHKKTYALKQLADFKAKIIENQFSGLHLDIDNEDVYFKLVGSFNAYNLLA
VYGTAILLEQDKLKVLTLLSRLSGAEGRFDYITSADKIIGIVDYAHTPDAVQNVLSTIAN
IRKGTEQVITVIGCGGDRDKTKRPIMAQVACDWSDKVILTSDNPRTEDAQTIINDMEAGV
SPTNKRKTLSILDRREAIKTACHLAQPGDIILVAGKGHEKYQEINGVRNHFDDKEILLEQ
LKPIS