Protein Info for CA265_RS00605 in Pedobacter sp. GW460-11-11-14-LB5

Annotation: endonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 TIGR01083: endonuclease III" amino acids 8 to 195 (188 residues), 241.1 bits, see alignment E=3.8e-76 PF00730: HhH-GPD" amino acids 35 to 169 (135 residues), 86.3 bits, see alignment E=2.6e-28 PF00633: HHH" amino acids 101 to 128 (28 residues), 25.8 bits, see alignment (E = 1.1e-09) PF10576: EndIII_4Fe-2S" amino acids 188 to 204 (17 residues), 21 bits, see alignment (E = 5.2e-08)

Best Hits

Swiss-Prot: 48% identical to END3_RICFE: Endonuclease III (nth) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K10773, endonuclease III [EC: 4.2.99.18] (inferred from 86% identity to phe:Phep_3556)

MetaCyc: 43% identical to endonuclease III (Escherichia coli K-12 substr. MG1655)
DNA-(apurinic or apyrimidinic site) lyase. [EC: 4.2.99.18]; 4.2.99.18 [EC: 4.2.99.18]; 4.2.99.18 [EC: 4.2.99.18]

Predicted SEED Role

"Endonuclease III (EC 4.2.99.18)" in subsystem Control of cell elongation - division cycle in Bacilli or DNA Repair Base Excision (EC 4.2.99.18)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1X9YZY4 at UniProt or InterPro

Protein Sequence (221 amino acids)

>CA265_RS00605 endonuclease III (Pedobacter sp. GW460-11-11-14-LB5)
MLKKERYKLFVEHFSAKQPDAETELHYHNPYQLLVAVILSAQCTDKRVNMVTPALFQRFP
NAKALAEATPDIVFDYIRSISYPNNKAKHLVGMANILLNEFNDVVPSGIDDLQKMPGVGR
KTANVIASVIYNAPAMAVDTHVFRVANRLGLTNGKTPLAVEKDLVKNLPEHDIHIAHHWL
ILHGRYVCVARSPKCEVCEITYMCRYFERLTAQNERDKLKA