Protein Info for BWI76_RS26425 in Klebsiella michiganensis M5al

Annotation: zinc/cadmium/mercury/lead-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 734 transmembrane" amino acids 129 to 146 (18 residues), see Phobius details amino acids 153 to 170 (18 residues), see Phobius details amino acids 188 to 215 (28 residues), see Phobius details amino acids 356 to 382 (27 residues), see Phobius details amino acids 388 to 412 (25 residues), see Phobius details amino acids 687 to 705 (19 residues), see Phobius details amino acids 710 to 731 (22 residues), see Phobius details PF00403: HMA" amino acids 55 to 111 (57 residues), 44.6 bits, see alignment 3.1e-15 TIGR01512: cadmium-translocating P-type ATPase" amino acids 185 to 732 (548 residues), 535.5 bits, see alignment E=1.8e-164 TIGR01525: heavy metal translocating P-type ATPase" amino acids 185 to 730 (546 residues), 587.3 bits, see alignment E=5.9e-180 TIGR01494: HAD ATPase, P-type, family IC" amino acids 208 to 707 (500 residues), 262.6 bits, see alignment E=8e-82 PF00122: E1-E2_ATPase" amino acids 236 to 416 (181 residues), 160.7 bits, see alignment E=5.6e-51 PF00702: Hydrolase" amino acids 434 to 641 (208 residues), 116.4 bits, see alignment E=4.5e-37 PF08282: Hydrolase_3" amino acids 624 to 674 (51 residues), 25.5 bits, see alignment 2.1e-09

Best Hits

Swiss-Prot: 77% identical to ZNTA_SHISS: Zinc/cadmium/lead-transporting P-type ATPase (zntA) from Shigella sonnei (strain Ss046)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 88% identity to kpe:KPK_0278)

MetaCyc: 77% identical to Zn2+/Cd2+/Pb2+ exporting P-type ATPase (Escherichia coli K-12 substr. MG1655)
3.6.3.3-RXN [EC: 7.2.2.21]; RXN0-5205 [EC: 7.2.2.21, 7.2.2.12]; 7.2.2.- [EC: 7.2.2.21, 7.2.2.12]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.12 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAV8 at UniProt or InterPro

Protein Sequence (734 amino acids)

>BWI76_RS26425 zinc/cadmium/mercury/lead-transporting ATPase (Klebsiella michiganensis M5al)
MSAPESQDKKVPHFSSLKFTPAAQPAESCCADHACSSETTAENDALSHARFSWLVEGMDC
AACARKVETAVRQVPGVSQVQVLFATEKLLVNAEGDVGPQIESAVRAAGYQLRDENAPRT
EDATSWLRENLPLITLVAMMALSWGLEQFNPPLGEIAFIATTLVGLWPVARQAIRLIKSG
SWFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEGWAASRARQGVSALMALKPDT
AIRVRDGVRETVAQRDLRPGDVIEVAAGGRLPADGQLLSPFASFDESALTGESVPVERNA
GERVAAGATSVDRLVQLTVISEPGDSAIDRILKLIEEAEERRAPIERFIDRFSRIYTPAI
MVVALLVAIVPPLLFAGAWLPWIYKGLTLLLIGCPCALVISTPAAITSGLAVAARRGALI
KGGAALEQLGRVRQVAFDKTGTLTIGQPRVTAVMATTDIGENDLLALAAAVEQGSSHPLA
QAVVREAEQRQLVIPPASGQRALAGSGIEAQVEGRQVLICAAGKNNDPQYDARIQQLESA
GQTVVLVMRDETLLGILALRDTLRDDARQAVDELHQLGVQGVILTGDNPRAAAAIASELG
LAFRAGLLPADKVQAVMALNAESPLAMVGDGINDAPAMKAATIGIAMGSGTDVALETADA
ALTHNRLTGLAQMISLARATHANIRQNIAIALGLKGIFLVTTLLGLTGLWLAVLADTGAT
VLVTANALRLLRKK