Protein Info for BWI76_RS26160 in Klebsiella michiganensis M5al

Updated annotation (from data): 4-alpha-glucanotransferase (EC 2.4.1.25)
Rationale: Specifically important for utilizing a-Cyclodextrin. Automated validation from mutant phenotype: the predicted function (2.4.1.25) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 697 PF21226: MalQ_N" amino acids 54 to 129 (76 residues), 61.7 bits, see alignment E=8.2e-21 TIGR00217: 4-alpha-glucanotransferase" amino acids 101 to 686 (586 residues), 908.3 bits, see alignment E=1.3e-277 PF02446: Glyco_hydro_77" amino acids 149 to 665 (517 residues), 481.7 bits, see alignment E=2.5e-148

Best Hits

Swiss-Prot: 83% identical to MALQ_ECOLI: 4-alpha-glucanotransferase (malQ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to eae:EAE_05340)

MetaCyc: 83% identical to 4-alpha-glucanotransferase (Escherichia coli K-12 substr. MG1655)
4-alpha-glucanotransferase. [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; 2.4.1.25 [EC: 2.4.1.25]; MALTODEG-RXN [EC: 2.4.1.25]; RXN0-7347 [EC: 2.4.1.25]

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAR4 at UniProt or InterPro

Protein Sequence (697 amino acids)

>BWI76_RS26160 4-alpha-glucanotransferase (EC 2.4.1.25) (Klebsiella michiganensis M5al)
METKRLDNAALAAGISPSYINAHGKPQSIAAATKQRLLDAMHRTTVTPKVAVNPLPAVQV
FTHGKKMSLPVAGQGEFHWILTTEEGKQYQGQARGGETLPLPARLPEGYHTLTLTQDGDR
WHCRAIVAPARCYEPQALKDGQKLWGTCVQLYTLRSEKNWGIGDFGDLRAMLPEIARRGG
SFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNAVEDFRHSKEAQKWWHSPATQQ
TLQAARQTDDVDYTAVTTLKLTALRMAWKGFSARNDEAMAEFRQFVTREGDSLYWQAAFD
ALHAWQVKQDPMRWGWPAWPKAYQDTDSPEVKAFCAEHADEVNFYLWLQWLAYTQFAECW
HTSQNDSMPIGLYRDLAVGVAEGGAETWCDRELYCLKASVGAPPDILGPLGQNWGLPPMD
PHIMVARAYEPFVELLRANMQNCGALRIDHVMSVLRLWWIPYGETADHGAYVQYPVDDLL
SILALESKRHRCMVIGEDLGTVPVEIVGKLRKSGVYSYKVLYFENDHEKTFRAPKAYPEQ
SMAVATTHDLPTLRGYWESGDLTLGKSLGLYPDEVVLRGLYQDRELAKQGLLDALHRYGC
LPKRTGHKASLMAMTPTLNRGMQRYIADSNSALLGLQPEDWLVMAEPVNIPGTSTEYPNW
RRKLSTTLEAMFADEQVNKLIKDLDKRRKAASKKKPA