Protein Info for BWI76_RS25315 in Klebsiella michiganensis M5al
Annotation: glutamate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)
MetaCyc: 95% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285BAP7 at UniProt or InterPro
Protein Sequence (1486 amino acids)
>BWI76_RS25315 glutamate synthase large subunit (Klebsiella michiganensis M5al) MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQ KPDRFFRIVAEERGWRLAKNYAVGMLFLNKDPELAKAARRIVEEELQRETLSIVGWRDVP TNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIARRRIEKRLQEDKDFYVCSLS NLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPFRYLAHNGE INTITGNRQWARARTYKFQTPLIPDLHDAAPFVNETGSDSSSMDNMLELLLAGGMDIIRA MRLLVPPAWQNNPDMDPDLRSFFDFNSMHMEPWDGPAGIVMSDGRYAACNLDRNGLRPAR YVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTREGRILHSAETDNDLKSRH PYKEWMAKNVRRLVPFEDLPDEEVGSRQLDDDTLASYQKQFNYSAEELDSVLRVLGENGQ EAVGSMGDDTPFAVLSSQPRIIYDYFRQQFAQVTNPPIDPLREAHVMSLATSIGREMNVF CEAEGQAHRLSFKSPILLYSDFKQLTTMEEEHYRADVLDITFDATATTLAETVKALCDKA EQMVRNGTVLLVLSDRNIAKNRLPVPAPMAVGAIQTRLVDKSLRCDANIIVETASARDPH HFAVLLGFGATAIYPYLAYETLAKLVDNKAIDKEYRAVMLNYRNGINKGLYKIMSKMGIS TIASYRCSKLFEAVGLHRDVSDLCFQGVVSRIGGAGFDDFQQDLLNLSKRAWLARKPLDQ GGLLKYVHGGEYHAYNPDVVRTLQQAVQSGEYGDYQQYAKLVNERPAATLRDLLAVTPDG TTVSLEDVEPASELFKRFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYG TNKVSRIKQVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSV PGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADL ITIAGYDGGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGLDII KAAILGAESFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYF EFIARETRELMAQLGVKRLVDLIGRTDLLKELDGFTAKQQKLDLSKMLETAEPHPGKALY CTENNPPFDNGVLNAQLLQQAKPFVDSKQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQG LAADPIVAHFSGTAGQSFGVWNAGGVELHLTGDANDYVGKGMAGGLLAIRPPVGSAFRSH EASIIGNTCLYGATGGRLYAAGRAGERFAVRNSGAITVVEGIGDNGCEYMTGGIVCVLGK TGVNFGAGMTGGFGYVLDEDGDFRKRVNPELVEVLDVDSLAIHEEHLRGLITEHVQLTGS QRGEEILANWPAFSAKFALVKPKSSDVKALLGHRSRSAAELRVQAQ