Protein Info for BWI76_RS25300 in Klebsiella michiganensis M5al

Annotation: hybrid sensor histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 779 transmembrane" amino acids 20 to 46 (27 residues), see Phobius details amino acids 58 to 77 (20 residues), see Phobius details PF18415: HKR_ArcB_TM" amino acids 15 to 89 (75 residues), 105.2 bits, see alignment E=8.6e-34 TIGR00229: PAS domain S-box protein" amino acids 152 to 276 (125 residues), 53.9 bits, see alignment E=1e-18 PF00989: PAS" amino acids 156 to 266 (111 residues), 63.1 bits, see alignment E=8.2e-21 PF08448: PAS_4" amino acids 161 to 271 (111 residues), 53.9 bits, see alignment E=7.2e-18 PF00512: HisKA" amino acids 283 to 345 (63 residues), 63.7 bits, see alignment 4.8e-21 PF02518: HATPase_c" amino acids 395 to 506 (112 residues), 104.7 bits, see alignment E=1.4e-33 PF00072: Response_reg" amino acids 529 to 640 (112 residues), 76.3 bits, see alignment E=7.6e-25 PF01627: Hpt" amino acids 687 to 761 (75 residues), 45.3 bits, see alignment E=3e-15

Best Hits

Swiss-Prot: 87% identical to ARCB_ECOLI: Aerobic respiration control sensor protein ArcB (arcB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 94% identity to eae:EAE_04480)

MetaCyc: 87% identical to sensor histidine kinase ArcB (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Aerobic respiration control sensor protein arcB (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BA88 at UniProt or InterPro

Protein Sequence (779 amino acids)

>BWI76_RS25300 hybrid sensor histidine kinase/response regulator (Klebsiella michiganensis M5al)
MKQIRMLAQYYVDLMMKLGLVRFSMLLAFALVVLAIVVQMAVTMVLHGQVESIDVIRSIF
FGLLITPWAVYFLSVVVEQLEESRQRLSRLVEKLEEMRERDLKLNVQLKDNIAQLNQEIG
EREKAEAERETTLEQLKIEMKEREETQIQLEQQSSFLRSFLDASPDLVFYRNEDKEFSGC
NRAMELLTGKSEKQLIHLKPQDVYSEEVAEKVLETDEKVFRHNVSLTYEQWLDYPDGRKA
CFEIRKVPYYDRVGKRRGLMGFGRDITERKRYQDALERASRDKTTFISTISHELRTPLNG
IVGLSRILLDTDLTDEQERYLKTIHVSAVTLGNIFNDIIEMDKMERRKIQLDNQPVDFTG
FLADLENLSGLQAQQKGLRFVLDPSLPLPHKVITDGTRLRQILWNLISNAVKFTPQGGSV
NVRVRYDEGDILHFEVEDSGIGIPEAEQDKIFAMYYQVKDSQGGKPATGTGIGLAVSRRL
ARNMGGDISVASLPGKGATFTLTVHAPAVAEEVEDTLAEDEMPLPALNVLLVEDIELNVI
VARSVLEKLGNSVDVAMTGKAALEMFVPGEYDLVLLDIQLPDMTGLDISRQLKQRFAADE
LPPLVALTANVLKNKTEYLEAGMDDVLSKPLSVPALTAIIKKFWDAPEEEENEMPSVDIS
KSASVLDIPMLEQYIELVGPKLINDGLAVFERMMPGYMAVLESNLTARDQKGIVDEGHKI
KGAAGSIGLRHIQQLGQQIQTPDLPAWSDNVGEWVEEMKSEWQNDVAVLKAWVAAADKK