Protein Info for BWI76_RS24855 in Klebsiella michiganensis M5al

Annotation: tagatose-bisphosphate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 TIGR00167: ketose-bisphosphate aldolase" amino acids 1 to 283 (283 residues), 342.6 bits, see alignment E=1.9e-106 TIGR01858: class II aldolase, tagatose bisphosphate family" amino acids 3 to 283 (281 residues), 499.2 bits, see alignment E=2.7e-154 PF01116: F_bP_aldolase" amino acids 5 to 283 (279 residues), 345.4 bits, see alignment E=1.4e-107

Best Hits

Swiss-Prot: 96% identical to GATY_KLEOX: D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) from Klebsiella oxytoca

KEGG orthology group: K08302, tagatose 1,6-diphosphate aldolase [EC: 4.1.2.40] (inferred from 95% identity to kva:Kvar_0554)

MetaCyc: 65% identical to tagatose-1,6-bisphosphate aldolase 2 (Escherichia coli K-12 substr. MG1655)
Tagatose-bisphosphate aldolase. [EC: 4.1.2.40]

Predicted SEED Role

"Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)" in subsystem D-Tagatose and Galactitol Utilization or Lactose and Galactose Uptake and Utilization or N-Acetyl-Galactosamine and Galactosamine Utilization (EC 4.1.2.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.40

Use Curated BLAST to search for 4.1.2.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285BAF5 at UniProt or InterPro

Protein Sequence (284 amino acids)

>BWI76_RS24855 tagatose-bisphosphate aldolase (Klebsiella michiganensis M5al)
MFIISSKNMLLKAQRLGYAVPAFNIHNLETMQVVVETAAELRSPLILAGTPGTYSYAGTG
NVVAIARDLAKIWDLPLAVHLDHHEDLADITRKVQAGIRSVMIDGSHSPFEENVALVKSV
VELSHRYDASVEAELGRLGGVEDDLVVDAKDALYTNPEQAREFVARTGIDSLAVAIGTAH
GLYAAEPKLDFDRLAAIRDRVDVPLVLHGASGLPDSDIRRAISLGVCKVNVATELKIAFS
DALKHYFEENPDANDPRHYMKPAKAAMKDVVRKVIHVCGCEGQL