Protein Info for BWI76_RS24075 in Klebsiella michiganensis M5al

Annotation: glutathione synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 TIGR01380: glutathione synthase" amino acids 2 to 311 (310 residues), 464.2 bits, see alignment E=8.7e-144 PF02951: GSH-S_N" amino acids 2 to 120 (119 residues), 167.3 bits, see alignment E=2.7e-53 PF02955: GSH-S_ATP" amino acids 124 to 296 (173 residues), 269.2 bits, see alignment E=2.3e-84 PF14398: ATPgrasp_YheCD" amino acids 131 to 186 (56 residues), 29.4 bits, see alignment E=9.9e-11 PF08443: RimK" amino acids 135 to 309 (175 residues), 41 bits, see alignment E=3.4e-14

Best Hits

Swiss-Prot: 92% identical to GSHB_SALTY: Glutathione synthetase (gshB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 95% identity to eae:EAE_03115)

MetaCyc: 90% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B999 at UniProt or InterPro

Protein Sequence (315 amino acids)

>BWI76_RS24075 glutathione synthase (Klebsiella michiganensis M5al)
MIKLGIVMDPIATINIKKDTSFAMLLEAQRRGYELHYMEMNDLYLINGEARARTRKLSVE
QNYDKWYAFNGEQDLPLADLDVILMRKDPPFDTEFIYATYILERAEEQGTLIVNKPQSLR
DCNEKLFTAWFSELTPETLVTRNKAQLKAFWEKHGDIILKPLDGMGGTSIFRVKEGDPNL
GVITETLTELGNRYCMAQNYLPAIKDGDKRVLVVDGEPVPYCLARIPQGGETRGNLAAGG
RGEARPLTESDWAIARRVGPTLKAKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPIS
ITGMLMDAIEQRLNK