Protein Info for BWI76_RS23800 in Klebsiella michiganensis M5al

Annotation: peptide chain release factor 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 TIGR00020: peptide chain release factor 2" amino acids 2 to 340 (339 residues), 575.5 bits, see alignment E=2e-177 PF03462: PCRF" amino acids 8 to 196 (189 residues), 205.3 bits, see alignment E=8.4e-65 PF00472: RF-1" amino acids 205 to 314 (110 residues), 145 bits, see alignment E=9.4e-47

Best Hits

Swiss-Prot: 98% identical to RF2_KLEP3: Peptide chain release factor 2 (prfB) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 97% identity to eco:b2891)

Predicted SEED Role

"Peptide chain release factor 2; programmed frameshift-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B913 at UniProt or InterPro

Protein Sequence (342 amino acids)

>BWI76_RS23800 peptide chain release factor 2 (Klebsiella michiganensis M5al)
MFDYDAKKERLEEVNAELEQPDVWNEPERAQALGKERSSLEAIVDTLDQMSQGLEDVSGL
LELAVEADDEETFNEAVAELDVLEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQD
WASMLTRMYLRWAEARGFKTEIIEESEGEVAGIKSVTIKIIGDYAYGWLRTETGVHRLVR
KSPFDSGGRRHTSFSSAFVYPEVDDDIDIDINPADLRIDVYRASGAGGQHVNRTESAVRI
THIPTGLVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDTKSDIGWGSQI
RSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEASLKAGL