Protein Info for BWI76_RS23005 in Klebsiella michiganensis M5al

Annotation: bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to COBU_SALTY: Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02231, adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase [EC: 2.7.1.156 2.7.7.62] (inferred from 94% identity to kpe:KPK_0933)

MetaCyc: 86% identical to adenosylcobinamide-phosphate guanylyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Adenosylcobinamide-phosphate guanylyltransferase. [EC: 2.7.7.62]; Adenosylcobinamide kinase. [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]

Predicted SEED Role

"Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.7.7.62)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (31/36 steps found)
superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
adenosylcobinamide-GDP salvage from assorted adenosylcobamides (1/2 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.156, 2.7.7.62

Use Curated BLAST to search for 2.7.1.156 or 2.7.7.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7S6 at UniProt or InterPro

Protein Sequence (181 amino acids)

>BWI76_RS23005 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (Klebsiella michiganensis M5al)
MLTLVTGGARSGKSRHAEALIAHAPQVLYIATSQIFDDEMAARIQHHRDGRPAHWCTAER
WQQLDELITPDIAPQEAILLECITTMVTNLLFALGGDSAPDGWDYAAMERAIDAEIDVLI
AACQRCPARVVLVTNEVGMGIVPENRLARHFRDIAGRVNQRLAAAADDVWLVVSGIGVKI
K