Protein Info for BWI76_RS22695 in Klebsiella michiganensis M5al

Annotation: sulfate adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 26 to 232 (207 residues), 160.2 bits, E=4.6e-51
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 28 to 434 (407 residues), 692.8 bits, E=1.6e-212
• TIGR00231: small GTP-binding protein domain
  • amino acids 34 to 214 (181 residues), 51.3 bits, E=1.2e-17
• PF01926: MMR_HSR1
  • amino acids 86 to 169 (84 residues), 23.8 bits, E=4.1e-09

Best Hits

Swiss-Prot: 89% identical to CYSN_ECO55: Sulfate adenylyltransferase subunit 1 (cysN) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: None (inferred from 93% identity to eae:EAE_01895)

MetaCyc: 89% identical to sulfate adenylyltransferase subunit 1 (Escherichia coli K-12 substr. MG1655)
Sulfate adenylyltransferase. [EC: 2.7.7.4]

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4)" in subsystem Cysteine Biosynthesis (EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.7.4

Sequence Analysis Tools

See A0A285AXR3 at UniProt or InterPro

Protein Sequence (475 amino acids)

>BWI76_RS22695 sulfate adenylyltransferase (Klebsiella michiganensis M5al)
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAVNKMDLVDFSEETF
NTIREAYLTFAEQLPGDLDIRFVPLSALEGDNVASQSEKMPWYSGPTLLEVLETVEIQRV
VESQPLRFPVQYVNRPNLDFRGFSGTVAAGRVSVGQRLKVLPSGVESTVARIVTFDGDLP
EAAAGEAITLVLKDEIDISRGDLLVDAAVSLPAVQSASVDVVWMAEQALAPGQSYDIKIA
GKKTRARVDGVRYQVDINNLTQREVESLPLNGIGLVDLTFDEPLVLDKYQDNPVTGGLIF
IDRLTNVTVGAGMVREPNEQAQAGASQYSAFELELNQLIRKHFPHWDARDLLGGK