Protein Info for BWI76_RS22360 in Klebsiella michiganensis M5al

Annotation: hydrogenase formation protein HypD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 TIGR00075: hydrogenase expression/formation protein HypD" amino acids 2 to 370 (369 residues), 561.4 bits, see alignment E=4.2e-173 PF01924: HypD" amino acids 17 to 367 (351 residues), 495.8 bits, see alignment E=3.6e-153

Best Hits

Swiss-Prot: 86% identical to HYPD_ECOLI: Hydrogenase maturation factor HypD (hypD) from Escherichia coli (strain K12)

KEGG orthology group: K04654, hydrogenase expression/formation protein HypD (inferred from 93% identity to kpu:KP1_4334)

MetaCyc: 86% identical to Fe-(CN)2CO cofactor assembly scaffold protein HypD (Escherichia coli K-12 substr. MG1655)
RXN-22646; RXN-22647

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypD" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXJ7 at UniProt or InterPro

Protein Sequence (373 amino acids)

>BWI76_RS22360 hydrogenase formation protein HypD (Klebsiella michiganensis M5al)
MRYVDEYRAPEQVMQLVEHLRQRARLLPHTADRPLRIMEVCGGHTHAIFKFGLDQLLPEN
IEFIHGPGCPVCVLPMGRIDACIDIASRPGVIFCTFGDAMRVPGKNGSLLQAKSRGADVR
IVYSPMDALKLAQQNPEREVVFFGLGFETTMPATALTLRQARERGVANFYFFCQHITLLP
TLRSLLDQPDNGIDAFLAPGHVSMVIGTDAYGFIASDYHRPLVVAGFEPLDLLQGAVMLV
EQTIARHSDVENQYRRVVPDEGNPLAQSAIAEVFCLSGDSEWRGLGVISDSGVHLTAAYQ
RFDAEAHFRPAPQRVSDDPRARCGEVLTGRCKPHQCPLFGKTCNPQSAFGALMVSSEGAC
AAWYQYRSQEIEA