Protein Info for BWI76_RS22070 in Klebsiella michiganensis M5al

Annotation: proline/betaine ABC transporter permease ProW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 transmembrane" amino acids 96 to 118 (23 residues), see Phobius details amino acids 123 to 143 (21 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 192 to 219 (28 residues), see Phobius details amino acids 271 to 291 (21 residues), see Phobius details amino acids 303 to 321 (19 residues), see Phobius details PF00528: BPD_transp_1" amino acids 163 to 329 (167 residues), 98 bits, see alignment E=2.8e-32

Best Hits

Swiss-Prot: 91% identical to PROW_ECOLI: Glycine betaine/proline betaine transport system permease protein ProW (proW) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 94% identity to eae:EAE_01440)

MetaCyc: 91% identical to glycine betaine ABC transporter membrane subunit ProW (Escherichia coli K-12 substr. MG1655)
RXN-8638 [EC: 7.6.2.9]; 7.4.2.- [EC: 7.6.2.9]

Predicted SEED Role

"L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXG2 at UniProt or InterPro

Protein Sequence (354 amino acids)

>BWI76_RS22070 proline/betaine ABC transporter permease ProW (Klebsiella michiganensis M5al)
MADQSNPWDTAPAADSAAQSADAWGTSTPAPTNGGSADWLSSAPAPQPEHFNIMDPFHNT
LIPLDSWVTHGIDWVVMHFRPLFQGIRVPVDYILSAFQQLLLGMPAPVAIIIFSLIAWQI
SSVGMGAATLISLIAIGAIGAWSQAMVTLALVLTALLFCMLIGLPLGIWLARSPRAAKII
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIVRLTILGINQVPADLIEA
SRSFGASPRQLLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYATGPLGLITRPFCK