Protein Info for BWI76_RS21360 in Klebsiella michiganensis M5al

Annotation: L-aspartate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR00551: L-aspartate oxidase" amino acids 8 to 520 (513 residues), 757.9 bits, see alignment E=2.2e-232 PF00890: FAD_binding_2" amino acids 10 to 392 (383 residues), 349.5 bits, see alignment E=6.7e-108 PF01266: DAO" amino acids 10 to 208 (199 residues), 31.1 bits, see alignment E=3.8e-11 PF02910: Succ_DH_flav_C" amino acids 441 to 522 (82 residues), 55.7 bits, see alignment E=1e-18

Best Hits

Swiss-Prot: 88% identical to NADB_ECOLI: L-aspartate oxidase (nadB) from Escherichia coli (strain K12)

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 93% identity to kpu:KP1_4156)

MetaCyc: 88% identical to L-aspartate oxidase (Escherichia coli K-12 substr. MG1655)
1.5.98.-; L-aspartate oxidase. [EC: 1.4.3.16]

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7J6 at UniProt or InterPro

Protein Sequence (539 amino acids)

>BWI76_RS21360 L-aspartate oxidase (Klebsiella michiganensis M5al)
MKTTSDSSCDVLIIGSGAAGLSLALRLAERCTVTVLSKGPISEGSTFYAQGGIAAVFDET
DSVESHIEDTLIAGAGLCDRHAVSFVASNAKPCVQWLIDQGVLFDTQVQANGEESYHLTR
EGGHSHRRILHAADATGKAVETTLVSQALAHPNIRVLERSNAVDLIISDKIGLPGTRRVV
GAWIWNRNKEAVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGALLKRPDGTRFMPEFDERGELAPRDIVARAIDHE
MKRLGADCMYLDISHKPAEFIRHHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVD
DNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDIVKRMPFAQAVNELP
SWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRINMLQQELDEYYAR
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPEQLPDSGPSVLSPLAHIKR