Protein Info for BWI76_RS21115 in Klebsiella michiganensis M5al

Annotation: 3-mercaptopyruvate sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 transmembrane" amino acids 241 to 256 (16 residues), see Phobius details PF00581: Rhodanese" amino acids 9 to 128 (120 residues), 63.1 bits, see alignment E=1.5e-21 amino acids 165 to 271 (107 residues), 49.7 bits, see alignment E=2.2e-17

Best Hits

Swiss-Prot: 75% identical to THTM_ECOLI: 3-mercaptopyruvate sulfurtransferase (sseA) from Escherichia coli (strain K12)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 90% identity to kva:Kvar_1207)

MetaCyc: 75% identical to 3-mercaptopyruvate sulfurtransferase (Escherichia coli K-12 substr. MG1655)
3-mercaptopyruvate sulfurtransferase. [EC: 2.8.1.2]; Thiosulfate sulfurtransferase. [EC: 2.8.1.2, 2.8.1.1]

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7M3 at UniProt or InterPro

Protein Sequence (281 amino acids)

>BWI76_RS21115 3-mercaptopyruvate sulfurtransferase (Klebsiella michiganensis M5al)
MSTSFFVAADWLAEHIDDPEIQIIDARMAPPGQEQRDLNAEYQAGHIPGAVFFDIEALSD
HTSPLPHMMPRAEAFAVAMRELGVSSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSIVA
GGLEGWRRDELPLEQGLPELPEGDFDARLDPQAIKRLTDVLLVSHEGSAQIVDARPAGRF
NGQVAEPRPGLRSGHIPGALNVPWSELVINGELKTTDELNEIFARQGVDFEQPIIASCGS
GVTAAVVVLALTTLGVNGVSLYDGSWSEWGARTDLPIEPAQ