Protein Info for BWI76_RS21000 in Klebsiella michiganensis M5al

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 TIGR03706: exopolyphosphatase" amino acids 13 to 306 (294 residues), 321.5 bits, see alignment E=2.6e-100 PF02541: Ppx-GppA" amino acids 26 to 308 (283 residues), 344.9 bits, see alignment E=4.2e-107 PF21447: Ppx-GppA_III" amino acids 314 to 490 (177 residues), 222.4 bits, see alignment E=3.7e-70

Best Hits

Swiss-Prot: 86% identical to PPX_ECO57: Exopolyphosphatase (ppx) from Escherichia coli O157:H7

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 95% identity to kpe:KPK_1303)

MetaCyc: 86% identical to exopolyphosphatase (Escherichia coli K-12 substr. MG1655)
Exopolyphosphatase. [EC: 3.6.1.11]

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B7D0 at UniProt or InterPro

Protein Sequence (513 amino acids)

>BWI76_RS21000 exopolyphosphatase (Klebsiella michiganensis M5al)
MPINDNAPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLDENSVLSEE
AMTRGLNCLSLFAERLQGFDPSSVCIVGTHTLRQATNAAEFLKRAEQVIPYPIEIIPGNE
EARLIFMGVEHTQPERGRKLVIDIGGGSTELVIGEDFEPRLVESRRMGCVSFSQAYFAGG
VINKENFQRARLSAVQKLETLAWQFRIQGWNVALGASGTIKAAHEVLVAMGEKDGFITPE
RLEMLVEELLKYKNFDSLSLPGLSEDRKAVFAPGLAILCGVFDALAIKELRLSDGALREG
VLYEMEGRFRHQDIRSRTAQSLANQYNIDREQARRVLETTTHIFEQWQEQNPKLANPHLA
ALLKWATMLHEVGLNINHSGMHRHSAYILQNSDLPGFNQEQQMLMATLVRYHRKAIKLDD
LPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLKTEANHWTLTFPHDWFSQNA
LVLLDLEKEQEYWEGVPDWWLKISEEEKPDATS