Protein Info for BWI76_RS20150 in Klebsiella michiganensis M5al

Annotation: NADH:ubiquinone oxidoreductase subunit J

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00499: Oxidored_q3
  • amino acids 16 to 158 (143 residues), 132.4 bits, E=5.4e-43

Best Hits

Swiss-Prot: 92% identical to NUOJ_SHIFL: NADH-quinone oxidoreductase subunit J (nuoJ) from Shigella flexneri

KEGG orthology group: K00339, NADH dehydrogenase I subunit J [EC: 1.6.5.3] (inferred from 97% identity to kpu:KP1_3913)

MetaCyc: 92% identical to NADH:quinone oxidoreductase subunit J (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• nitrate reduction VIII (dissimilatory) (2/2 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See A0A285B7H4 at UniProt or InterPro

Protein Sequence (184 amino acids)

>BWI76_RS20150 NADH:ubiquinone oxidoreductase subunit J (Klebsiella michiganensis M5al)
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAIAGVFFSLGAYFAGALEIIVYA
GAIMVLFVFVVMMLNLGGSEIEQERQWLKPGIWIGPAILSAVLLVVIVYAILGVNDQGID
GAAINAKEVGIALFGPYVLAVELASMLLLAGLVVAFHIGREERAGEVLSNRPNDSDKRKT
EEHA