Protein Info for BWI76_RS19400 in Klebsiella michiganensis M5al

Annotation: NCS2 family permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 33 to 55 (23 residues), see Phobius details amino acids 67 to 88 (22 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 144 to 168 (25 residues), see Phobius details amino acids 181 to 199 (19 residues), see Phobius details amino acids 208 to 227 (20 residues), see Phobius details amino acids 247 to 265 (19 residues), see Phobius details amino acids 298 to 319 (22 residues), see Phobius details amino acids 331 to 353 (23 residues), see Phobius details amino acids 360 to 377 (18 residues), see Phobius details amino acids 389 to 416 (28 residues), see Phobius details amino acids 427 to 445 (19 residues), see Phobius details PF00860: Xan_ur_permease" amino acids 31 to 392 (362 residues), 103 bits, see alignment E=7.9e-34

Best Hits

Swiss-Prot: 34% identical to ADEQ_ECOLI: Adenine permease AdeQ (adeQ) from Escherichia coli (strain K12)

KEGG orthology group: K06901, putative MFS transporter, AGZA family, xanthine/uracil permease (inferred from 94% identity to enc:ECL_03426)

MetaCyc: 34% identical to adenine transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-577

Predicted SEED Role

"Xanthine/uracil/thiamine/ascorbate permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6S5 at UniProt or InterPro

Protein Sequence (447 amino acids)

>BWI76_RS19400 NCS2 family permease (Klebsiella michiganensis M5al)
MADNTLNSSAQGSWLERRFALYSRGSTLRTECLAGITGFLAAAYLLVVIPGLLAVGGMDK
GAATTGTILVFVGGTLLMAFYANLPFIVGPGIGGSVLVGVTLAGSEGIGWQVGLGIACWS
GILFFLLTKFGLREVVTRSVPQSIKLGLTASIGLFVAVLGFRNAGLVLANAKTNALMLGD
FLSPGALVALCGLFLAIALQARKIPGSILWAILFATLVGIPMGVTRLPGSFIDMPHSLTP
VLGQIDMLGALNIAFLPFLFVFFASEFFSTMGTTLAVGGEAGLLDEEGNMPHINRPFMVD
SIAAAVGPWLGIPAATALIESSAAAEAGGKTGLTALSAAVMFLLMLLFTPVALMIPKEAT
APALILIGLNMFSGLRKVDLANFTDGLPVLMMVMITLIANSFGTGIAGGLLFYVVIKTIA
GKWREIPIGLWILAIPLVYYFATLVKH