Protein Info for BWI76_RS19215 in Klebsiella michiganensis M5al

Annotation: proline-specific peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR01250: proline-specific peptidase" amino acids 5 to 293 (289 residues), 259 bits, see alignment E=3.2e-81 PF00561: Abhydrolase_1" amino acids 30 to 281 (252 residues), 91.6 bits, see alignment E=1.3e-29 PF12146: Hydrolase_4" amino acids 31 to 281 (251 residues), 54.8 bits, see alignment E=1.7e-18 PF12697: Abhydrolase_6" amino acids 31 to 283 (253 residues), 61.3 bits, see alignment E=4.5e-20 PF02129: Peptidase_S15" amino acids 97 to 275 (179 residues), 27.1 bits, see alignment E=6.5e-10

Best Hits

Swiss-Prot: 68% identical to LAAA_PSEFS: L-amino acid amidase (laaA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 83% identity to ddc:Dd586_2920)

MetaCyc: 66% identical to L-proline amide hydrolase (Pseudomonas azotoformans)
RXN-10757 [EC: 3.5.1.101]; 3.5.1.101 [EC: 3.5.1.101]

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5 or 3.5.1.101

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6N7 at UniProt or InterPro

Protein Sequence (310 amino acids)

>BWI76_RS19215 proline-specific peptidase (Klebsiella michiganensis M5al)
MYTISEGYAPFRRYQTWFRVCGDIDSGLTPLVIAHGGPGCTHDYVDAFRDIALSGRAVIH
YDQLGNGRSTHLPQQPADFWQPALFLDELANLLQHLNIADHYALLGQSWGGMLGAEHAVT
RPKGLKALIIANSPASMALWLQGAARLRAQLPADVQQTLLAHEAAGMLDSAEYKAASQVF
YQRHVCRLDPWPAEVRRTFDAMDADPTVYHAMNGPTEFHVIGSMKAWTIIDRLARIDVPV
LLISGRYDEATPEVVQPFMDNIPDARWVIFEASSHMPHVEERELCMKTVNDFLSRAINEK
PLTAPARLRR