Protein Info for BWI76_RS19055 in Klebsiella michiganensis M5al

Annotation: dTDP-4-dehydrorhamnose 3,5-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01221: dTDP-4-dehydrorhamnose 3,5-epimerase
  • amino acids 2 to 176 (175 residues), 270.6 bits, E=2.9e-85
• PF00908: dTDP_sugar_isom
  • amino acids 6 to 175 (170 residues), 248.7 bits, E=1.3e-78

Best Hits

Swiss-Prot: 63% identical to RMLC_SHIFL: dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Shigella flexneri

KEGG orthology group: K01790, dTDP-4-dehydrorhamnose 3,5-epimerase [EC: 5.1.3.13] (inferred from 82% identity to dze:Dd1591_2857)

MetaCyc: 58% identical to dTDP-4-dehydrorhamnose 3,5-epimerase (Escherichia coli K-12 substr. MG1655)
dTDP-4-dehydrorhamnose 3,5-epimerase. [EC: 5.1.3.13]

Predicted SEED Role

"dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)" in subsystem Capsular heptose biosynthesis or Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 5.1.3.13)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (9/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.13

Protein Sequence (178 amino acids)

>BWI76_RS19055 dTDP-4-dehydrorhamnose 3,5-epimerase (Klebsiella michiganensis M5al)
MQVIDTKIADVKIIQPKVFGDARGFFVETFEKKRYQEMLNIEFDFVQDNHSRSTKGVLRG
LHFQTQHAQGKLVRVVRGEVFDVAVDIRPESPTFGQWEGVILSEENKTQFWIPPGLAHGF
VVLSDIADFEYKCTDYYNPGFEGCILWNDVDIGIEWPEKNPLLSEKDKQGKTLKELFA