Protein Info for BWI76_RS19035 in Klebsiella michiganensis M5al

Annotation: dTDP-rhamnosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 PF00535: Glycos_transf_2" amino acids 8 to 133 (126 residues), 38.2 bits, see alignment E=1.4e-13 TIGR01556: rhamnosyltransferase" amino acids 9 to 287 (279 residues), 264.5 bits, see alignment E=4.8e-83 PF13632: Glyco_trans_2_3" amino acids 82 to 207 (126 residues), 31.4 bits, see alignment E=1.7e-11

Best Hits

Predicted SEED Role

"dTDP-rhamnosyl transferase RfbF (EC 2.-.-.-)" in subsystem dTDP-rhamnose synthesis (EC 2.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (295 amino acids)

>BWI76_RS19035 dTDP-rhamnosyl transferase (Klebsiella michiganensis M5al)
MKDLFAVIVTYHPQLINLKKLIVALREQGVSPVIVDNGTLKQDSLSEIENIAHVINLGDN
MGIAFAQNKGILYCKSKNAKAIVFFDQDSEIKAEFIESLVNDYNDLIAAGDKVAVIGPTF
IDSRYGFYYKQIRLNKFGIREKVDPAHFSKPFEATIIISSGSYIPVKVLDDVGYMDEDFF
IDYVDTEWCLRAISKGYKIFVSTNAVMEHAIGDKMINIGGLHVPVHSPIRRYYRIRNAIL
FSKLSHIPLMLKIRDNLMNIVHQLLLILTQKHKFLNARTMCKGIKDGFVGKVGKG