Protein Info for BWI76_RS19025 in Klebsiella michiganensis M5al

Annotation: phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)" amino acids 5 to 468 (464 residues), 758.7 bits, see alignment E=1.2e-232 PF03446: NAD_binding_2" amino acids 5 to 174 (170 residues), 170.8 bits, see alignment E=2.4e-54 PF00393: 6PGD" amino acids 179 to 466 (288 residues), 448.4 bits, see alignment E=1.2e-138

Best Hits

Swiss-Prot: 97% identical to 6PGD_KLEPN: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Klebsiella pneumoniae

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 98% identity to kva:Kvar_1562)

MetaCyc: 94% identical to 6-phosphogluconate dehydrogenase, decarboxylating (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B6J3 at UniProt or InterPro

Protein Sequence (468 amino acids)

>BWI76_RS19025 phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating) (Klebsiella michiganensis M5al)
MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIAENPGKKLVPHYTVQEF
VESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGF
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIAAVAEDGEPCVTYIGADGAGH
YVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQTFTEWNEGELSSYLIDITKDIFT
KKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLITESVFARYISSLKDQRVAAS
KVLTGPQAKSAGDKAEFVEKVRRALYLGKIVSYAQGFSQLRAASDEYHWDLNYGEIAKIF
RAGCIIRAQFLQKITNAYEQNAGIANLLLAPYFKQIADDYQQALRDVVAYAVQNGIPVPT
FSAAIAYYDSYRSAVLPANLIQAQRDYFGAHTYKRTDKEGVFHTEWMD