Protein Info for BWI76_RS18125 in Klebsiella michiganensis M5al

Annotation: murein DD-endopeptidase MepM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details PF08525: OapA_N" amino acids 12 to 39 (28 residues), 37.6 bits, see alignment (E = 3.8e-13) PF04225: OapA" amino acids 97 to 171 (75 residues), 30.1 bits, see alignment E=1.1e-10 PF01476: LysM" amino acids 99 to 142 (44 residues), 34.3 bits, see alignment 4.5e-12 PF19425: Csd3_N2" amino acids 180 to 301 (122 residues), 222.2 bits, see alignment E=3.2e-70 PF01551: Peptidase_M23" amino acids 313 to 407 (95 residues), 119.7 bits, see alignment E=1.2e-38

Best Hits

Swiss-Prot: 88% identical to MEPM_ECOLI: Murein DD-endopeptidase MepM (mepM) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to eae:EAE_22830)

MetaCyc: 88% identical to peptidoglycan endopeptidase MepM (Escherichia coli K-12 substr. MG1655)
3.4.-.-; 3.4.-.-

Predicted SEED Role

"Cell wall endopeptidase, family M23/M37"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B5X6 at UniProt or InterPro

Protein Sequence (440 amino acids)

>BWI76_RS18125 murein DD-endopeptidase MepM (Klebsiella michiganensis M5al)
MQQIARAVTQAFNNLPRPHRVMLGSLSVLTLAVAVWRPYIYHHPETAPISRTIELEQSEI
RSLLPEASEPIDQAPQEEEAIPQDELDDKADSDSGGHEYVVSTGDTLSSILNQYGIDMGD
IARLSSVDKELRNLKIGQQLSWTLTADGDLQSLTWEMSRRETRTYDRVANGFKMSSELQK
GDWVNSVLKGTVGGSFVASARDAGLTSTEISAVIKAMQWQMDFRKLKKGDEFSVLMSREM
LDGKREQSQLLGVRLRSDGKDYYAIRAEDGKFYDRNGTGLAKGFLRFPTARQFRVSSNFN
PRRLNPVTGRVAPHRGVDFAMPQGTPVLSVGDGEVVVAKRSGAAGYYVAVRHGRTYTTRY
MHLRKLLVKPGQKVKRGDRIALSGNTGRSTGPHLHYEVWINQQAVNPLTAKLPRTEGLSG
SDRTDYLAQAKQVIPQLRFD