Protein Info for BWI76_RS18000 in Klebsiella michiganensis M5al
Annotation: 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to RSMF_KLEP3: Ribosomal RNA small subunit methyltransferase F (rsmF) from Klebsiella pneumoniae (strain 342)
KEGG orthology group: None (inferred from 87% identity to eae:EAE_22700)MetaCyc: 78% identical to 16S rRNA m5C1407 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11593 [EC: 2.1.1.178]
Predicted SEED Role
"Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-)" (EC 2.1.1.-)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.- or 2.1.1.178
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B5P9 at UniProt or InterPro
Protein Sequence (491 amino acids)
>BWI76_RS18000 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF (Klebsiella michiganensis M5al) MVNYAPFFPCGALPVAQNAVYLPEEFLAQMRSAMPTHLSFDDFIAACQRPLRRSIRVNTL KITVADFLQLVAPYRWQLTPVPWCEEGFWIEREDEDDLPLGSTAEHLSGLFYIQEASSML PVAALFADGETPQRLMDVAAAPGSKTTQIAARMGNEGGILANEYSASRVKVLHANISRCG ISNVALTHFDGRVFGAALPECFDAILLDAPCSGEGVVRKDPDALKNWSPESNAEIALTQR ELIDSAFHALRPGGMLVYSTCTLNRQENEEVVNGLLARYPQAVQVLPLGSLFPQASEALT DEGFLHVFPQIFDCEGFFVARLRKTAAIEPLPAPGYKVGKFPFTPLKTRESAAVIAAAAA VGLQWSAEHTLWQRDKEIWLFPQALEALFGKVRFSRIGIRLAETHNKGYRWQHEAVIALA APAAPFALTQAEAEEWYRGRDVYPETLPEKDDAIVTYQGAPLGLAKKVGSRLKNSYPREL VRDGKLFSKKA