Protein Info for BWI76_RS17890 in Klebsiella michiganensis M5al
Annotation: PTS mannose transporter subunit EIIAB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to PTNAB_SHIFL: PTS system mannose-specific EIIAB component (manX) from Shigella flexneri
KEGG orthology group: K02793, PTS system, mannose-specific IIA component [EC: 2.7.1.69] K02794, PTS system, mannose-specific IIB component [EC: 2.7.1.69] (inferred from 96% identity to kpu:KP1_3458)MetaCyc: 94% identical to mannose-specific PTS enzyme IIAB component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-157 [EC: 2.7.1.199]; 2.7.1.199 [EC: 2.7.1.199]; TRANS-RXN-167 [EC: 2.7.1.199, 2.7.1.193]; TRANS-RXN-165 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]; 2.7.1.191 [EC: 2.7.1.199, 2.7.1.193, 2.7.1.191]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
- glycolysis I (from glucose 6-phosphate) (13/13 steps found)
- D-mannose degradation I (2/2 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.69
Use Curated BLAST to search for 2.7.1.191 or 2.7.1.193 or 2.7.1.199 or 2.7.1.69
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B5F7 at UniProt or InterPro
Protein Sequence (322 amino acids)
>BWI76_RS17890 PTS mannose transporter subunit EIIAB (Klebsiella michiganensis M5al) MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTAK GVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDNPSFDELVALAVE TGREGVKALKAKPVEKAAPAPVQAAAPKAAAPLKPMGPNDYMVIGLARIDDRLIHGQVAT RWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERI MLLFTNPTDVERVVEGGVKITSVNIGGMAFRQGKTQVNNAISVDEKDIEAFKKLNARGIE LEARKVSTDQKLKMMDLIGKVK