Protein Info for BWI76_RS17095 in Klebsiella michiganensis M5al

Annotation: vitamin B12 ABC transporter ATP-binding protein BtuD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 PF00005: ABC_tran" amino acids 18 to 162 (145 residues), 76.5 bits, see alignment E=3.3e-25

Best Hits

Swiss-Prot: 68% identical to BTUD_CITK8: Vitamin B12 import ATP-binding protein BtuD (btuD) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 80% identity to eae:EAE_17045)

MetaCyc: 65% identical to vitamin B12 ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-5-RXN [EC: 7.6.2.8]; 7.6.2.8 [EC: 7.6.2.8]

Predicted SEED Role

"Vitamin B12 ABC transporter, ATPase component BtuD" in subsystem Coenzyme B12 biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B501 at UniProt or InterPro

Protein Sequence (249 amino acids)

>BWI76_RS17095 vitamin B12 ABC transporter ATP-binding protein BtuD (Klebsiella michiganensis M5al)
MPFLMQLQDVEEAGRLAPFSADIRPGEIIHLVGPNGAGKSTLLARMAGLSTGPGRVKLNE
RPLKDWPAQELARHRAYLSQHQMPPFAMPVWHYLSLHLHNDAQNALLTAIAEKLGLGDKL
GRQVNHISGGEWQRVRLAAVILQIHRQANPVGQLLLLDEPMNSLDVAQQSALDRVLNELC
ASGVAIVMSSHDLNHTLRHAHRAWLICRGKTIACGSTAEVLNEENLTAAYAMDFQRVEVA
GHLMLVPSQ