Protein Info for BWI76_RS16635 in Klebsiella michiganensis M5al

Annotation: carbonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00484: Pro_CA
  • amino acids 34 to 188 (155 residues), 161.1 bits, E=1.3e-51

Best Hits

Swiss-Prot: 66% identical to CYNT_ECOLI: Carbonic anhydrase 1 (cynT) from Escherichia coli (strain K12)

KEGG orthology group: K01673, carbonic anhydrase [EC: 4.2.1.1] (inferred from 95% identity to kpu:KP1_3090)

MetaCyc: 66% identical to carbonic anhydrase 1 (Escherichia coli K-12 substr. MG1655)
Carbonate dehydratase. [EC: 4.2.1.1]

Predicted SEED Role

"Carbonic anhydrase (EC 4.2.1.1)" in subsystem Carboxysome or Cyanate hydrolysis (EC 4.2.1.1)

MetaCyc Pathways

• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
• cyanate degradation (2/3 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (23/56 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.1

Use Curated BLAST to search for 4.2.1.1

Sequence Analysis Tools

See A0A285B4S2 at UniProt or InterPro

Protein Sequence (211 amino acids)

>BWI76_RS16635 carbonate dehydratase (Klebsiella michiganensis M5al)
MQHIIEGFLNFQKEIFPQRKELFRSLASSQNPKALFISCSDSRLVPELVTQQEPGQLFVI
RNAGNIVPSFGPEPGGVSATIEYAVVALGVTDIVICGHSNCGAMKAIATCQCLDPMPAVS
HWLRYSDAAKAVVEKKTWANETDKVNAMVQENVIAQLNNIKTHPSVAVGLRDSTLRLHGW
FYDIESGDIRALDKNSKTFVSLSENPDVYFE