Protein Info for BWI76_RS16485 in Klebsiella michiganensis M5al

Annotation: ribonuclease T

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01298: ribonuclease T
  • amino acids 10 to 208 (199 residues), 360.9 bits, E=8.9e-113
• PF00929: RNase_T
  • amino acids 20 to 194 (175 residues), 104.5 bits, E=4.4e-34

Best Hits

Swiss-Prot: 97% identical to RNT_KLEP7: Ribonuclease T (rnt) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K03683, ribonuclease T [EC: 3.1.13.-] (inferred from 97% identity to kpn:KPN_01991)

MetaCyc: 86% identical to ribonuclease T (Escherichia coli K-12 substr. MG1655)
Ribonuclease D. [EC: 3.1.13.5]; 3.1.13.5 [EC: 3.1.13.5]; 3.1.13.- [EC: 3.1.13.5]

Predicted SEED Role

"Ribonuclease T (EC 3.1.13.-)" in subsystem tRNA processing (EC 3.1.13.-)

MetaCyc Pathways

• tRNA processing (10/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.5

Use Curated BLAST to search for 3.1.13.- or 3.1.13.5

Sequence Analysis Tools

See A0A285B4R4 at UniProt or InterPro

Protein Sequence (214 amino acids)

>BWI76_RS16485 ribonuclease T (Klebsiella michiganensis M5al)
MSENVQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEEGWLMPDETLHF
HVEPFEGANLQPEALAFNGINPHDPERGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAH
NATFDHSFTMAAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMAFDGTQA
HSALYDTEQTAQLFCEIVNRWKRLGGWPLPVAEE