Protein Info for BWI76_RS15425 in Klebsiella michiganensis M5al

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 transmembrane" amino acids 14 to 35 (22 residues), see Phobius details amino acids 52 to 71 (20 residues), see Phobius details amino acids 81 to 101 (21 residues), see Phobius details amino acids 108 to 130 (23 residues), see Phobius details amino acids 148 to 167 (20 residues), see Phobius details amino acids 173 to 191 (19 residues), see Phobius details amino acids 225 to 244 (20 residues), see Phobius details amino acids 265 to 288 (24 residues), see Phobius details amino acids 296 to 315 (20 residues), see Phobius details amino acids 321 to 342 (22 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details amino acids 386 to 405 (20 residues), see Phobius details PF01306: LacY_symp" amino acids 7 to 412 (406 residues), 711.5 bits, see alignment E=4.7e-218 TIGR00882: oligosaccharide:H+ symporter" amino acids 12 to 406 (395 residues), 678.8 bits, see alignment E=1.2e-208 PF12832: MFS_1_like" amino acids 18 to 388 (371 residues), 76.8 bits, see alignment E=2.6e-25 PF07690: MFS_1" amino acids 18 to 318 (301 residues), 83.9 bits, see alignment E=1.6e-27

Best Hits

Swiss-Prot: 99% identical to LACY_KLEOX: Lactose permease (lacY) from Klebsiella oxytoca

KEGG orthology group: None (inferred from 94% identity to eae:EAE_18470)

MetaCyc: 62% identical to lactose permease (Escherichia coli K-12 substr. MG1655)
RXN-17755; RXN0-7215; RXN0-7217; TRANS-RXN-24; TRANS-RXN-94

Predicted SEED Role

"Lactose permease" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Lactose and Galactose Uptake and Utilization or Lactose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B496 at UniProt or InterPro

Protein Sequence (416 amino acids)

>BWI76_RS15425 MFS transporter (Klebsiella michiganensis M5al)
MKLSELAPRERHNFIYFMLFFFFYYFIMSAYFPFFPVWLAEVNHLTKTETGIVFSCISLF
AIIFQPVFGLISDKLGLRKHLLWTITILLILFAPFFIFVFSPLLQMNIIAGALVGGVYLG
IVFSSGSGAVEAYIERVSRANRFEYGKVRVSGCVGWALCASITGILFSIDPNITFWIASG
FALILGVLLWVSKPESSNSAEVIDALGANRQAFSMRTAAELLRMPRFWGFIIYVVGVASV
YDVFDQQFANFFKGFFSSPQRGTEVFGFVTTGGELLNALIMFCAPAIINRIGAKNALLIA
GLIMSVRILGSSFATSAVEVIILKMLHMFEIPFLLVGTFKYISSAFNPKLSATLFLIGFN
LSKQLSGVVLSAWVGRMYDTVGFHQAYLILGCITLSFTVISLFTLKGSKTLLPATA