Protein Info for BWI76_RS15105 in Klebsiella michiganensis M5al

Annotation: 4-hydroxyphenylacetate 3-monooxygenase oxygenase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 PF11794: HpaB_N" amino acids 10 to 274 (265 residues), 292.8 bits, see alignment E=2.9e-91 PF03241: HpaB" amino acids 282 to 482 (201 residues), 235.5 bits, see alignment E=3.2e-74

Best Hits

Swiss-Prot: 62% identical to NPHA1_RHOSO: 4-nitrophenol 2-monooxygenase, oxygenase component (nphA1) from Rhodococcus sp.

KEGG orthology group: K00483, 4-hydroxyphenylacetate-3-hydroxylase large chain [EC: 1.14.13.3] (inferred from 74% identity to pay:PAU_00189)

MetaCyc: 62% identical to 4-nitrophenol 2-monooxygenase oxygenase component (Rhodococcus sp. PN1)
4-nitrophenol 2-monooxygenase. [EC: 1.14.13.29]

Predicted SEED Role

"4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.14.13.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.3

Use Curated BLAST to search for 1.14.13.29 or 1.14.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B4J2 at UniProt or InterPro

Protein Sequence (504 amino acids)

>BWI76_RS15105 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (Klebsiella michiganensis M5al)
MGNKSVSLPTGNDFLAGLNDGREIWAYGEKVDNVITHPAFRNSALSIAKLYDALHDEKYQ
AILTTPCDTGSGGVTHPFFRVPHSVDDLIRDRDAIAQWARLSYGWLGRSPDFKASFLTTL
GANPEAYGEYADNARHWYKRSQEELLYWNHAIINPPIDRNLPPDQIEDICIHVEKECDDG
VIVSGAKVVATGSALTHYNFIAHYGLPVKKREFALVCTLPMNAPGLKLVCRPSYSLAAEK
IGSPFDYPLSSRYDENDMIFILDKVKIPWENIFIYGDTAKAATFLPSSGFLHRSTFHGVT
RLAVKIDFICGLFIKGVEATGVADFRGIQTRVGEMLAWRNLFWAISDAMAKSPNPWHNGA
LLPNLDYGLAYRWFMSLGYPRIREIIEQDLGSALIYINSHAKDFAAPELEPYLKKYMRGS
NGMDAVERVKLMKLIWDSVGTEFASRHELYERNYTGSHENVRIELLLAAQMSGQVQEYKG
FAEQCMMDYDLDGWLDRDYHWPVD