Protein Info for BWI76_RS14770 in Klebsiella michiganensis M5al
Annotation: PTS IIA 2 domain protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 37% identical to ULAC_ECOL6: Ascorbate-specific PTS system EIIA component (ulaC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K02821, PTS system, ascorbate-specific IIA component [EC: 2.7.1.69] (inferred from 46% identity to lmh:LMHCC_2883)MetaCyc: 36% identical to L-ascorbate specific PTS enzyme IIA component (Escherichia coli K-12 substr. MG1655)
RXN0-2461 [EC: 2.7.1.194]
Predicted SEED Role
"Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-)" in subsystem L-ascorbate utilization (and related gene clusters) (EC 2.7.1.-)
MetaCyc Pathways
- L-ascorbate degradation I (bacterial, anaerobic) (5/5 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Nucleotide sugars metabolism
- Pentose phosphate pathway
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.1.69
Use Curated BLAST to search for 2.7.1.- or 2.7.1.194 or 2.7.1.69
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B4C0 at UniProt or InterPro
Protein Sequence (147 amino acids)
>BWI76_RS14770 PTS IIA 2 domain protein (Klebsiella michiganensis M5al) MIERQAINVDCAANSPEEAIREAGNALCRAGACSPQYVQAMVDSYRELGPYFVIAPGLAL PHARPEQGAMKAQISAIRLREPLAFGHAENDPVQVVLGLSATSSDAHIRLIQHIVTVLSD ENNLHTLMHSRDPEQLYRLLASGSQRL