Protein Info for BWI76_RS14200 in Klebsiella michiganensis M5al

Annotation: protocatechuate 4,5-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02900: LigB
  • amino acids 8 to 267 (260 residues), 206.5 bits, E=4.4e-65
• PF07746: LigA
  • amino acids 323 to 409 (87 residues), 105.3 bits, E=1.5e-34

Best Hits

Swiss-Prot: 71% identical to GALA_PSEPK: Gallate dioxygenase (galA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04099, protocatechuate 4,5-dioxygenase [EC: 1.13.11.8] (inferred from 94% identity to cro:ROD_31621)

MetaCyc: 71% identical to gallate dioxygenase monomer (Pseudomonas putida KT2440)
GALLATE-DIOXYGENASE-RXN [EC: 1.13.11.57]

Predicted SEED Role

"Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8)" in subsystem Central meta-cleavage pathway of aromatic compound degradation (EC 1.13.11.8)

MetaCyc Pathways

• gallate degradation I (4/4 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (6/8 steps found)
• 3,4-dichlorobenzoate degradation (3/4 steps found)
• superpathway of vanillin and vanillate degradation (7/10 steps found)
• 3-chlorobenzoate degradation II (via protocatechuate) (3/6 steps found)
• syringate degradation (6/12 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.57 or 1.13.11.8

Protein Sequence (420 amino acids)

>BWI76_RS14200 protocatechuate 4,5-dioxygenase (Klebsiella michiganensis M5al)
MARILGGIAVSHTPTIGFAVDHHKQQDPAWSPIFQSFEPLQRWLDEKKPDALVYIFNDHV
TAFFFDHYSAFTLGIDKEYGVADEGGGPRDLPPVKGDAALSRHIGASLMADEFDMSFFMN
KKLDHGLFSPLSALLPWQQEEGWPTCVIPLQIGVLQFPVPSAKRCYKLGQALRRAIESYP
EDIDVAIVATGGLSHQVHGERCGFNNTDWDAQFVDMLVNDPEKLTEMTISEYATLGGMEG
SEVIMWLVMRGALSANVTETWRDYYLPSMTGIATLILENNARTPPVDTLIRHRQHIAQQL
TGVDNLPGTYPFTHERSLNGLRLNGFLHKLTQPAWRERFLSTPQALYEEAELSLEEQRLL
TERDWRGLIRYGASFFLLEKMGAVVGVSNLHIYAAMRGQTLEEFQKTRNQQVTYSVAGTR