Protein Info for BWI76_RS13990 in Klebsiella michiganensis M5al
Annotation: allophanate hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 88% identity to kpe:KPK_2627)Predicted SEED Role
"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)
MetaCyc Pathways
- allantoin degradation IV (anaerobic) (8/9 steps found)
- uracil degradation III (5/5 steps found)
- L-citrulline degradation (3/3 steps found)
- urea degradation I (3/3 steps found)
- superpathway of allantoin degradation in yeast (5/6 steps found)
- L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
- cyanate degradation (2/3 steps found)
- cyanuric acid degradation II (3/5 steps found)
- cyanuric acid degradation I (2/5 steps found)
- superpathway of atrazine degradation (3/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.54
Use Curated BLAST to search for 3.5.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B3U6 at UniProt or InterPro
Protein Sequence (598 amino acids)
>BWI76_RS13990 allophanate hydrolase (Klebsiella michiganensis M5al) MMQQTFDLRLATLAADYRNGKTTPRRLLAEVRQRAQALNPEFRLFIHILSEEEQEPLLAA LEGLSPTELPLYGVPFAIKDNIDLAEIITTAACPAFAYRAEQSATIVAQLIALGAVPVGK TNLDQFATGLNGTRSPYGACRNGYLADYPSGGSSAGSALAVALGVASFSLGTDTAGSGRV PAGLNNLVGLKATKGLISTAGVVPACRTLDCVTFFSATADEASQLLALVAQYDPLDAWSR HNPQWNSRQAFGRPAAGFRFGVPRELNFLGCSASERLFTQARQRLTALGGVAVEIEFAPF LAAARLLYDGPWVAERYAVVGPLMARQPEAVLPVIREVLAKAPDTDATATFKAIYQLQQY KARCDAILETLDCVLTPTYPRPVTLDELQAEPIARNADLGYYTNFMNLLDYAAVSVPCGF MPDGLPSGVTLFGRAFTDQYLLSLADAFQRAERLPLAGGTRLETPPPERAAGHDRMALVV CGAHLAGLPLNDQLLARGGQLLQETRSAPHYRLYALADGKRPAMVRDESGGAAIAVEVWE LPSAEVGSLLAAIPAPLGLGKVELADGRRLTGFICEAEGLGEATEITAYGGWRAWLAR