Protein Info for BWI76_RS12135 in Klebsiella michiganensis M5al

Annotation: C-5 cytosine-specific DNA methylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 PF00145: DNA_methylase" amino acids 8 to 124 (117 residues), 23.1 bits, see alignment E=2.4e-09 amino acids 590 to 647 (58 residues), 23.9 bits, see alignment 1.3e-09

Best Hits

KEGG orthology group: K00558, DNA (cytosine-5-)-methyltransferase [EC: 2.1.1.37] (inferred from 87% identity to esc:Entcl_1848)

Predicted SEED Role

"FIG00731669: hypothetical protein"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.37

Use Curated BLAST to search for 2.1.1.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (660 amino acids)

>BWI76_RS12135 C-5 cytosine-specific DNA methylase (Klebsiella michiganensis M5al)
MRESEIIVDNFAGGGGASTGIELAIVRSVDIAINHDLNAVAMHTTNHPDTLHYCESVYSV
RPKVATAGRRVGLAWFSPDCRHFSKAKGAKPVEKAIRGLAWIVLRWGLDVDPRVMMLENV
EEFKTWGPLLAAEMRPDPDRVGETFRAFVGMLTTGIPANHPALAECCEFLNISLDSEDAA
RLVKGLGYIVEYRELRACDYGAPTIRKRFFMVMRCDGKPIVWPEATHGDPKSPAVLVGKL
APWRTAAECIDWSIPAPSIFDRKKPLAVNTLKRIARGIQRFVIDSASPFIVKCNHTTTKG
KYDCFRGQSLDEPLQTITKTHGYALAVPHLTKFRTGATGQPVTDPVPTVTAGTSKRPRGN
GHALGIVEAALAPFLAGNGGSEYQAKPRPLDKPAHTILKESRACVVAPVIARQFGASVGH
RVDEPSAIITAGGGGKSQLVVPTLIQMGYGERPGQEPRVLQLGNPLGTVTAGGNKFATVS
AFLAKHYGGNYQGAGVGLDEPTHSVTTVDHHAVVASHLVKLRGTCRDGQRTDAPMLTITA
GGQHVGEVKTTLAIEDYDEERAQQVLAFLQQYCGEDSNGLVDIGGVTYRIVDIGMRMLQP
HELYRAQGFPEWYIIDQDYRGVKYAKDKQVARCGNAVPPPFAEALVRANLPEMCRAREAA