Protein Info for BWI76_RS11410 in Klebsiella michiganensis M5al

Annotation: leucine efflux protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 212 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 68 (25 residues), see Phobius details amino acids 78 to 99 (22 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 151 to 178 (28 residues), see Phobius details amino acids 191 to 209 (19 residues), see Phobius details PF01810: LysE" amino acids 18 to 209 (192 residues), 179.1 bits, see alignment E=3.6e-57

Best Hits

Swiss-Prot: 92% identical to LEUE_KLEP7: Leucine efflux protein (leuE) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K11250, leucine efflux protein (inferred from 91% identity to kva:Kvar_3190)

MetaCyc: 74% identical to leucine exporter (Escherichia coli K-12 substr. MG1655)
RXN0-7050; TRANS-RXN0-270

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B2C3 at UniProt or InterPro

Protein Sequence (212 amino acids)

>BWI76_RS11410 leucine efflux protein (Klebsiella michiganensis M5al)
MFAEFGVLNYLTYLVGAIFIILVPGPNTFFVLKTGVAHGVRKGYLAAAGIFIGDAVLMFL
AFAGVATLIKTTPVLFNIVRYLGAIYLLWMGGKMLYAVLTQRDGHSSADSEPGSAILKRS
LTLSLTNPKAILFYVSFFVQFIDVSAKTPGLAFFILALTLEIISFIYMSFLILSGSFVTR
YVKTKKKLAKIGNSLIGLVFVGFAARLATLQS