Protein Info for BWI76_RS11255 in Klebsiella michiganensis M5al

Annotation: putrescine/spermidine ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF00005: ABC_tran" amino acids 34 to 175 (142 residues), 132.2 bits, see alignment E=3.1e-42 TIGR01187: polyamine ABC transporter, ATP-binding protein" amino acids 48 to 372 (325 residues), 543.1 bits, see alignment E=1.1e-167 PF08402: TOBE_2" amino acids 292 to 372 (81 residues), 30.4 bits, see alignment E=5.3e-11

Best Hits

Swiss-Prot: 90% identical to POTA_SHIDS: Spermidine/putrescine import ATP-binding protein PotA (potA) from Shigella dysenteriae serotype 1 (strain Sd197)

KEGG orthology group: K11072, spermidine/putrescine transport system ATP-binding protein [EC: 3.6.3.31] (inferred from 95% identity to kpe:KPK_3420)

MetaCyc: 90% identical to spermidine preferential ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.31

Use Curated BLAST to search for 3.6.3.31 or 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B2K6 at UniProt or InterPro

Protein Sequence (378 amino acids)

>BWI76_RS11255 putrescine/spermidine ABC transporter ATP-binding protein (Klebsiella michiganensis M5al)
MGQSQKLNTQPRSRSPLVQLAGIRKSFDGKTVISDLNLTINNGEFLTLLGPSGCGKTTVL
RLIAGLESVDSGRIHLEDHDITHIPAENRHVNTVFQSYALFPHMTVFENVAFGLRMQKTP
AAEITPRVHDALRMVQLDAFAQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDY
KLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRIVVMREGKIEQDGTPREIYEEPK
NLFVASFIGEINIFDATVIERIDEQRVRASVEGRECNIYVSFAVEKGQRLNVLLRPEDLR
VDEIHHSNDADGLIGYIRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKM
AINWVESWEVVLADEEHK