Protein Info for BWI76_RS10780 in Klebsiella michiganensis M5al
Annotation: pyrimidine utilization protein A
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to RUTA_KLEVT: Pyrimidine monooxygenase RutA (rutA) from Klebsiella variicola (strain At-22)
KEGG orthology group: K09018, putative monooxygenase RutA [EC: 1.14.-.-] (inferred from 96% identity to kpe:KPK_3520)MetaCyc: 92% identical to pyrimidine monooxygenase RutA (Escherichia coli K-12 substr. MG1655)
RXN-12886 [EC: 1.14.99.46]; 1.14.99.46 [EC: 1.14.99.46]
Predicted SEED Role
"Predicted monooxygenase RutA in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization
MetaCyc Pathways
- uracil degradation III (5/5 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Ascorbate and aldarate metabolism
- Benzoxazinone biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of steroids
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Carotenoid biosynthesis - General
- Diterpenoid biosynthesis
- Limonene and pinene degradation
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Sphingolipid metabolism
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
- gamma-Hexachlorocyclohexane degradation
Isozymes
Compare fitness of predicted isozymes for: 1.14.-.-
Use Curated BLAST to search for 1.14.-.- or 1.14.99.46
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B261 at UniProt or InterPro
Protein Sequence (363 amino acids)
>BWI76_RS10780 pyrimidine utilization protein A (Klebsiella michiganensis M5al) MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYGFDFALSMIKLRGFGGKTEF WDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMASTIDSISGGRFGVNLVTGW QKPEYEQMGMWPGDDYFASRYDYLTEYVQVLRDLWGTGRSDFKGDYFTMNDCRVSPQPSQ PMKVICAGQSDAGMAFSAQHADYNFCFGKGVNTPAAFAPTAARMMQAAEKTGRDVGSYVL FMVIADETDEAARAKWEHYKAGADDEALAWLTEQSQKDTRSGSDTNVRQMADPTSAVNIN MGTLVGSYASVARMLDEVAAVPGTDGVLLTFDDFLIGIEAFGQRIQPLMRCRDHIATMTQ EVA