Protein Info for BWI76_RS10590 in Klebsiella michiganensis M5al
Annotation: class V aminotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to PUCG_BACSU: (S)-ureidoglycine--glyoxylate transaminase (pucG) from Bacillus subtilis (strain 168)
KEGG orthology group: K00839, aminotransferase [EC: 2.6.1.-] (inferred from 92% identity to kpe:KPK_2608)MetaCyc: 92% identical to ureidoglycine aminotransferase subunit (Klebsiella pneumoniae pneumoniae MGH 78578)
RXN-12221
Predicted SEED Role
"Serine--pyruvate aminotransferase (EC 2.6.1.51) / L-alanine:glyoxylate aminotransferase (EC 2.6.1.44)" in subsystem Photorespiration (oxidative C2 cycle) or Pyruvate Alanine Serine Interconversions or Serine-glyoxylate cycle (EC 2.6.1.44, EC 2.6.1.51)
MetaCyc Pathways
- glycine biosynthesis III (1/1 steps found)
- L-serine biosynthesis II (3/4 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminophosphonate metabolism
- Arginine and proline metabolism
- Caprolactam degradation
- Glycine, serine and threonine metabolism
- Lysine biosynthesis
- Lysine degradation
- Methionine metabolism
- Nucleotide sugars metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.-
Use Curated BLAST to search for 2.6.1.- or 2.6.1.44 or 2.6.1.51
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285B1U7 at UniProt or InterPro
Protein Sequence (413 amino acids)
>BWI76_RS10590 class V aminotransferase (Klebsiella michiganensis M5al) MDITQFSQLNPPSRLLMGPGPINADPRVLRAMSSQLIGQYDPAMTHYMNEVMALYRGVFR TENRWTMLVDGTSRAGIEAILVSAIRPGDKVLVPVFGRFGHLLCEIARRCRAEVHTIEVP WGEVFTPDQVEDAVKRIRPRLLLTVQGDTSTTMLQPLAELGEICRRYDALFYTDATASLG GNPLETDVWGLDAVSAGMQKCLGGPSGTSPITLSARMEEAIRRRKCVEEGIRTDAHRDGD EEMIYSNYFDLGMVMDYWGPERLNHHTEATTALFGARECARLILQEGLDYGIARHKLHGD ALVKGIQAMGLETFGDLKHKMNNVLGVVIPQGINGDQARKLMLEDFGIEIGTSFGPLHGK VWRIGTMGYNARKDCVMTTLSALESVLNYLKFPTTQGAAMQAAWDHYRSERAQ