Protein Info for BWI76_RS09960 in Klebsiella michiganensis M5al

Annotation: Lon protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 transmembrane" amino acids 151 to 169 (19 residues), see Phobius details PF13654: AAA_32" amino acids 125 to 224 (100 residues), 52.3 bits, see alignment E=6.1e-18 PF05362: Lon_C" amino acids 435 to 535 (101 residues), 39.4 bits, see alignment E=4.9e-14

Best Hits

Swiss-Prot: 74% identical to LONH_ECOLI: Putative Lon protease homolog (ycbZ) from Escherichia coli (strain K12)

KEGG orthology group: K04770, Lon-like ATP-dependent protease [EC: 3.4.21.-] (inferred from 86% identity to kva:Kvar_3401)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type II" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-, 3.4.21.53

Use Curated BLAST to search for 3.4.21.- or 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1I3 at UniProt or InterPro

Protein Sequence (585 amino acids)

>BWI76_RS09960 Lon protease (Klebsiella michiganensis M5al)
MTITKLTRNDLAPDTESYQALFAQADLAHAEESLSGELQPRLFYALEQLLVTPAISPFML
VKIPEEPEYLQWLANETRTLHQPAATLCGVHYQVDGGKISLTPAQTAEDNFASVAPVEAA
DWIEAEQLFGCVRQFNGEITLQAGLVHRANGGVLILSLRALLAQPLLWMRLKNMVARQRF
DWLSFDESRPLPVSIPSMPLSLKVILVGERESLADFQEMEPELSAQAIYSEYEDNLQIAD
AETLKQWCQWVWQNAQQLELPGLSANAWPLLIQEGARYTGDQETLPLCVLWIARQLREAA
AFCDGEEISAEEIQTMLVRRLWREGYLAERIQDEILQEQILIETEGECVGQINALSVIEF
PGHPRAFGEPSRISCVVHIGDGEFIDVERKAELGGNIHAKGMMIMQAFLMSELELEQQLP
FTASLTFEQSYSEVDGDSASMAELCALISSLANVPINQSIAVTGSVDQFGRVQPVGGLNE
KIEGFFAICQQRGLTGKQGVIIPSANVRHLSLAQELQQAVSDEQFFIWAVDDVSEALPIL
TQLLWDGEGQTLRQTIQERIAQATQQESRHRFPWPLRWLGGSGSN