Protein Info for BWI76_RS09160 in Klebsiella michiganensis M5al

Annotation: multidrug transporter MdfA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 transmembrane" amino acids 15 to 33 (19 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 142 to 164 (23 residues), see Phobius details amino acids 171 to 189 (19 residues), see Phobius details amino acids 219 to 240 (22 residues), see Phobius details amino acids 257 to 275 (19 residues), see Phobius details amino acids 287 to 308 (22 residues), see Phobius details amino acids 313 to 337 (25 residues), see Phobius details amino acids 347 to 367 (21 residues), see Phobius details amino acids 378 to 399 (22 residues), see Phobius details PF07690: MFS_1" amino acids 25 to 364 (340 residues), 101.9 bits, see alignment E=3.7e-33 PF00083: Sugar_tr" amino acids 58 to 194 (137 residues), 26 bits, see alignment E=4.4e-10

Best Hits

Swiss-Prot: 86% identical to MDFA_ECO57: Multidrug transporter MdfA (mdfA) from Escherichia coli O157:H7

KEGG orthology group: K08160, MFS transporter, DHA1 family, multidrug/chloramphenicol efflux transport protein (inferred from 91% identity to kva:Kvar_3503)

MetaCyc: 86% identical to multidrug efflux pump MdfA / Na+:H+ antiporter / K+:H+ antiporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-101; TRANS-RXN-337; TRANS-RXN-338; TRANS-RXN-42; TRANS-RXN-44

Predicted SEED Role

"Multidrug translocase MdfA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285B1F7 at UniProt or InterPro

Protein Sequence (411 amino acids)

>BWI76_RS09160 multidrug transporter MdfA (Klebsiella michiganensis M5al)
MQNYSLSGKRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLSVVAEFGVGNEWVPTSMTA
YLAGGMFLQWLLGPLSDRIGRRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLC
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWVHLLPWEMMFVLFAVL
AAIAFFGLQKAMPETATRMGEKLSVKELGRDYREVLKNLRFVAGALATGFVSLPLLAWIA
QSPVIIISGEQATSYEYGLLQVPIFGALIAGNLVLARQTSRKTVRSLIILGGWPIMIGLL
ISAVATVASTHAYLWMTAGLSVYAFGIGLANAGLVRLTLFASEMSKGTVSAAMGMLQMLI
FTVGIEVSKHAYEFGGSGLFSLLNLLSGVLWLAMTVYFLKDKRVGNAHEPQ